Antibody Testing Data:
FLISA plate was coated with purified human IgA, IgG, and IgM. Immunoglobulins were detected with serially diluted Goat F(ab’)2 Anti-Human IgA-AF555 (SB Cat. No. 2052-32).
Product Details
Product Specifications |
|
|---|---|
|
Name: |
Goat F(ab’)2 Anti-Human IgA-Alexa Fluor® 555 |
| Clone: |
Polyclonal |
|
Isotype: |
Goat F(ab')2 IgG ( Isotype Control : -) |
|
Specificity: |
Reacts with the heavy chain of human IgA |
| Format/Conjugate: |
AF555 (Alexa Fluor® 555) |
| Buffer Formulation: |
Phosphate buffered saline containing < 0.1% sodium azide |
|
Concentration: |
0.5 mg/mL |
|
Volume: |
1.0 mL |
|
Storage & Handling: |
2-8°C; Avoid exposure to light blue Please refer to product specific SDS. |
| RRID: |
AB_2795712 |
|
Recommended Dilutions: |
Please refer to product specific Technical Guide. |
|
Applications: |
Quality tested applications for relevant formats include - |
|
More information: |
Source:Pepsin digest of Goat Anti-Human IgA (SB Cat. No. 2050) |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Documents
Technical Guide
Technical Guide: Goat F(ab’)2 Anti-Human IgA-Alexa Fluor® 555
Safety Data Sheets (SDS)
SDS: Goat F(ab’)2 Anti-Human IgA-Alexa Fluor® 555 References
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2. Matousovic K, Novak J, Yanagihara T, Tomana M, Moldoveanu Z, Kulhavy R, et al. IgA-containing immune complexes in the urine of IgA nephropathy patients. Nephrol Dial Transplant. 2006;21:2478-84. (ELISA)
3. Huggins J, Pellegrin T, Felgar RE, Wei C, Brown M, Zheng B, et al. CpG DNA activation and plasma-cell differentiation of CD27- naive human B cells. Blood. 2007;109:1611-9. (ELISA)
4. Berthelot L, Papista C, Maciel TT, Biarnes-Pelicot M, Tissandie E, Wang PH, et al. Transglutaminase is essential for IgA nephropathy development acting through IgA receptors. J Exp Med. 2012;209:793-806. (ELISA)
5. Sira MM, Yoshida T, Takeuchi M, Kashiwayama Y, Futatani T, Kanegane H, et al. A novel immunoregulatory protein in human colostrum, syntenin-1, for promoting the development of IgA-producing cells from cord blood B cells. Int Immunol. 2009;21:1013-23. (ELISpot)
6. White CA, Pone EJ, Lam T, Tat C, Hayama KL, Li G, et al. Histone deacetylase inhibitors upregulate B cell microRNAs that silence AID and Blimp-1 expression for epigenetic modulation of antibody and autoantibody responses. J Immunol. 2014;193:5933-50. (ELISA)
7. Boumediene A, Oblet C, Oruc Z, Duchez S, Morelle W, Huynh A, et al. Gammopathy with IgA mesangial deposition provides a monoclonal model of IgA nephritogenicity and offers new insights into its molecular mechanisms. Nephrol Dial Transplant. 2011;26:3930-7. (IHC-FS)
8. Burgener A, Tjernlund A, Kaldensjo T, Abou M, McCorrister S, Westmacott GR, et al. A systems biology examination of the human female genital tract shows compartmentalization of immune factor expression. J Virol. 2013;87:5141-50. (IHC-FS)
9. Grossetête B, Launay P, Lehuen A, Jungers P, Bach J, Monteiro RC. Down-regulation of Fcα receptors on blood cells of IgA nephropathy patients: evidence for a negative regulatory role of serum IgA. Kidney Int. 1998;53:1321-35. (FC)
10. Morton HC, van Zandbergen G, van Kooten C, Howard CJ, van de Winkel JG, Brandtzaeg P. Immunoglobulin-binding sites of human FcαRI (CD89) and bovine Fcγ2R are located in their membrane-distal extracellular domains. J Exp Med. 1999;189:1715-22. (FC)
11. Janoff EN, Fasching C, Orenstein JM, Rubins JB, Opstad NL, Dalmasso AP. Killing of Streptococcus pneumoniae by capsular polysaccharide-specific polymeric IgA, complement, and phagocytes. J Clin Invest. 1999;104:1139-47. (FC)
12. van der Pol W, Vidarsson G, Vilé HA, van de Winkel JG, Rodriguez ME. Pneumococcal capsular polysaccharide-specific IgA triggers efficient neutrophil effector functions via FcαRI (CD89). J Infect Dis. 2000;182:1139-45. (FC)
13. Rodriguez ME, Hellwig SM, Hozbor DF, Leusen J, van der Pol W, van de Winkel JG. Fc receptor-mediated immunity against Bordetella pertussis. J Immunol. 2001;167:6545-51. (FC)
14. Honorio-Franca AC, Launay P, Carneiro-Sampaio MM, Monteiro RC. Colostral neutrophils express Fcα receptors (CD89) lacking γ chain association and mediate noninflammatory properties of secretory IgA. J Leukoc Biol. 2001;69:289-96. (FC)
15. Heystek HC, Moulon C, Woltman AM, Garonne P, van Kooten C. Human immature dendritic cells efficiently bind and take up secretory IgA without the induction of maturation. J Immunol. 2002;168:102-7. (FC)
16. Mota G, Manciulea M, Cosma E, Popescu I, Hirt M, Jensen-Jarolim E, et al. Human NK cells express Fc receptors for IgA which mediate signal transduction and target cell killing. Eur J Immunol. 2003;33:2197-205. (FC)
17. Toapanta FR, Bernal PJ, Sztein MB. Diverse phosphorylation patterns of B cell receptor-associated signaling in naïve and memory human B cells revealed by phosphoflow, a powerful technique to study signaling at the single cell level. Front Cell Infect Microbiol. 2012;2:128. (Stim)
Alexa Fluor® is a registered trademark of Life Technologies Corporation
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