Donkey Anti-Goat IgG(H+L), Rat/Mouse SP ads-BIOT



Datasheet      Tech Support









Antibody Testing Data:

Frozen newborn mouse cartilage section was stained with Goat Anti-Type II Collagen-UNLB (SB Cat. No. 1320-01; right) followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-BIOT (SB Cat. No. 6420-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.








Product Details






Product Specifications

Name:

Donkey Anti-Goat IgG(H+L), Rat/Mouse SP ads-BIOT

Clone:

Polyclonal

Isotype:

Donkey IgG (Isotype Control: -)

Specificity:

Reacts with the heavy and light chains of goat IgG

Format/Conjugate:

BIOT (Biotin)

Buffer Formulation:

Phosphate buffered saline containing 0.1% sodium azide

Concentration:

0.5 mg/mL

Volume:

2.0 mL

Storage & Handling:

2-8°C blue Please refer to product specific SDS.

RRID:

AB_2796336

Recommended Dilutions:

Please refer to product specific Technical Guide.

Applications:

Quality tested applications for relevant formats include -
ELISA 1
FLISA
Other referenced applications for relevant formats include -
Flow Cytometry 2,3
Immunohistochemistry-Frozen Sections 4
Immunohistochemistry-Paraffin Sections 11
Western Blot 5-9
Multiplex 10

More information:

Source: Pooled antisera from rabbits hyperimmunized with goat IgG.
Cross Adsorption: Mouse and rat serum proteins (SP); may react with immunoglobulins from other species and the light chains of other goat immunoglobulins.
Purification: Affinity chromatography on goat IgG covalently linked to agarose.

*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
















References






1. Migliore MM, Ortiz R, Dye S, Campbell RB, Amiji MM, Waszczak BL. Neurotrophic and neuroprotective efficacy of intranasal GDNF in a rat model of Parkinson's disease. Neuroscience. 2014;274:11-23. (ELISA)

2. Rasche SS, Phillips M, McInerney MF, Sercarz EE, Quinn A. IL-13Rα1 expression on β-cell-specific T cells in NOD mice. Diabetes. 2011;60:1716-25. (FC)

3. Biedroń R, Konopiński MK, Marcinkiewicz J, Józefowski S. Oxidation by neutrophils-derived HOCl increases immunogenicity of proteins by converting them into ligands of several endocytic receptors involved in antigen uptake by dendritic cells and macrophages. PLoS One. 2015;10(4) :e0123293. (FC)

4. Xia RH, Yosef N, Ubogu EE. Selective expression and cellular localization of pro-inflammatory chemokine ligand/receptor pairs in the sciatic nerves of a severe murine experimental autoimmune neuritis model of Guillain-Barré syndrome. Neuropathol Appl Neurobiol. 2010;36:388-98. (IHC-FS)

5. Liu S, Bubar MJ, Lanfranco MF, Hillman GR, Cunningham KA. Serotonin2C receptor localization in GABA neurons of the rat medial prefrontal cortex: implications for understanding the neurobiology of addiction. Neuroscience. 2007;146:1677-88. (WB)

6. Kumar GR, Shum L, Glaunsinger BA. Importin α-mediated nuclear import of cytoplasmic poly(A) binding protein occurs as a direct consequence of cytoplasmic mRNA depletion. Mol Cell Biol. 2011;31:3113-25. (WB)

7. Gao H, Yallampalli U, Yallampalli C. Maternal protein restriction reduces expression of angiotensin I-converting enzyme 2 in rat placental labyrinth zone in late pregnancy. Biol Reprod. 2011;86:31. (WB)

8. Gao H, Yallampalli U, Yallampalli C. Protein restriction to pregnant rats increases the plasma levels of angiotensin II and expression of angiotensin II receptors in uterine arteries. Biol Reprod. 2012;86:68. (WB)

9. Bouchez I, Pouteaux M, Canonge M, Genet M, Chardot T, Guillot A, et al. Regulation of lipid droplet dynamics in Saccharomyces cerevisiae depends on the Rab7-like Ypt7p, HOPS complex and V1-ATPase. Biol Open. 2015;00:pii: bio.20148615. (WB)

10. Yang G, Holl TM, Liu Y, Li Y, Lu X, Nicely NI, et al. Identification of autoantigens recognized by the 2F5 and 4E10 broadly neutralizing HIV-1 antibodies. J Exp Med. 2013;210:241-56. (Multiplex)

11. SouthernBiotech published data (IHC-PS)










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