Catalog#: 31-1001-02
Applications: WB (nonreduced), IP, ICC, IHC, FC, ELISA
Price Not Available
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Product Details
Product Specifications |
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Product Name |
Biotin Anti-Mouse IgG rabbit monoclonal antibody [RM104] |
Product Description: |
Biotinylated Rabbit monoclonal to Mouse IgG |
Catalog No. |
31-1001-02 |
Clone Name |
RM104 |
Product Datasheet |
|
Specificity |
This antibody reacts to the Fc region of all subclasses of mouse IgG. No cross reactivity with mouse IgM, IgA, IgE, human IgG, and rat IgG. It may cross react to goat IgG. |
Immunogen |
Mouse IgG |
Application |
WB (nonreduced), IP, ICC, IHC, FC, ELISA |
Species Reactivity |
Mouse |
Conjugate |
Biotin |
Intended Use |
Research Use Only |
Properties |
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Host |
Rabbit |
Storage Instruction |
Store at -20.0°C |
Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
|
Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
Clone Type |
Recombinant Monoclonal |
Isotype |
Rabbit IgG |
Uniport |
|
Gene ID |
16017 |
Usage |
ELISA: 0.005 ug/mL-0.2 ug/mL;Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;Western Blot (WB): 0.1 ug/mL-0.5 ug/mL. |
Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Western blot of nonreduced(-) and reduced(+) mouse immunoglobulins (20ng/lane), using 0.2ug/mL of RevMAb clone RM104. This antibody reacts to nonreduced mouse IgG1, IgG2a, IgG2b, and IgG3. It showed no cross reactivity with IgM, IgA, or IgE.
ELISA of mouse immunoglobulins shows RM104 reacts to the Fc region of mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM104 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of RM104 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.