Catalog#: 31-1025-02
Applications: ICC, IHC, FC, ELISA
Price Not Available
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Product Details
Product Specifications |
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|---|---|
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Product Name |
Biotin Anti-Human IgD rabbit monoclonal antibody [RM123] |
| Product Description: |
Biotinylated Rabbit monoclonal to Human IgD |
|
Catalog No. |
31-1025-02 |
|
Clone Name |
RM123 |
| Product Datasheet |
|
| Specificity |
This antibody reacts to human IgD. No cross reactivity with human IgG, IgM, IgA, or IgE. |
|
Immunogen |
Human IgD |
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Application |
ICC, IHC, FC, ELISA |
|
Species Reactivity |
Human |
| Conjugate |
Biotin |
|
Intended Use |
Research Use Only |
Properties |
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|---|---|
|
Host |
Rabbit |
|
Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
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| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
|
Clone Type |
Recombinant Monoclonal |
|
Isotype |
Rabbit IgG |
|
Uniport |
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|
Gene ID |
3495 |
| Usage |
ELISA: 25ng/well – 200ng/well (for Capture);0.01ug/mL – 0.1ug/mL (for Detection);Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL. |
|
Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Sandwich ELISA using RM123 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
ELISA of human immunoglobulins shows RM123 reacts to IgD from human plasma and IgDκ from human myeloma. No cross reactivity with human IgG, IgM, IgA, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM123 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA using RM123. The plate was coated with different amounts of human IgD (from plasma). A serial dilution of RM123 was used as the primary antibody. An alkaline phosphatase conjugated anti- rabbit IgG as the secondary antibody.