Biotin Anti-Human Ig Light Chains rabbit monoclonal antibody [RM129]
Datasheet
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Catalog#: 32-1031-02
Applications: ICC, IHC, FC, ELISA
Price Not Available
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Product Details
Product Specifications |
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|---|---|
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Product Name |
Biotin Anti-Human Ig Light Chains rabbit monoclonal antibody [RM129] |
| Product Description: |
Biotinylated Rabbit monoclonal to Human Immunoglobulin Light Chains |
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Catalog No. |
32-1031-02 |
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Clone Name |
RM129 |
| Product Datasheet |
Biotin Anti-Human Ig Light Chains rabbit monoclonal antibody [RM129] |
| Specificity |
This antibody reacts to both kappa and lambda light chains of human immunoglobulins. The antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. |
|
Immunogen |
Human IgG |
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Application |
ICC, IHC, FC, ELISA |
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Species Reactivity |
Human |
| Conjugate |
None |
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Intended Use |
Research Use Only |
Properties |
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|---|---|
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Host |
Rabbit |
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Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
Biotin Anti-Human Ig Light Chains rabbit monoclonal antibody [RM129] |
| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
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Clone Type |
Recombinant Monoclonal |
|
Isotype |
Rabbit IgG |
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Uniport |
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Gene ID |
3514/3537 |
| Usage |
ELISA: 0.1 ug/mL – 0.5 ug/mL;Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL. |
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Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
ELISA showing RM129 reacts only to kappa and lambda light chain of all human immunoglobulins, not to mouse IgG, rat IgG, or goat IgG.
Detection of human IgG1 in monkey serum, using RM117 (capture) and biotin-RM129 (detection) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.
ELISA showing RM129 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM129 and a monkey IgG binding antibody (positive control) was used as the detection antibody.