Catalog#: 31-1048-02
Applications: WB, IP, ICC, FC , IHC
Price Not Available
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Product Details
Product Specifications |
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|---|---|
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Product Name |
Biotin Anti-His-Tag rabbit monoclonal antibody [RM146] |
| Product Description: |
Biotinylated Rabbit monoclonal to His-Tag (6xHis-Tag or 10xHis-Tag) |
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Catalog No. |
31-1048-02 |
|
Clone Name |
RM146 |
| Product Datasheet |
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| Specificity |
This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells. |
|
Immunogen |
Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus. |
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Application |
WB, IP, ICC, FC , IHC |
|
Species Reactivity |
All Species |
| Conjugate |
Biotin |
|
Intended Use |
Research Use Only |
Properties |
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|---|---|
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Host |
Rabbit |
|
Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
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| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
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Clone Type |
Recombinant Monoclonal |
|
Isotype |
Rabbit IgG |
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Uniport |
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|
Gene ID |
N/A |
| Usage |
Western Blot: 0.1 ug/mL – 0.5 ug/mL;Immunocytochemistry 0.5 ug/mL – 2 ug/mL;Flow Cytometry: 0.5 ug/mL – 2 ug/mL;Immunohistochemistry; 0.1 ug/mL-1 ug/mL;ELISA: 0.01 ug/mL-0.5 ug/mL. |
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Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
A titer ELISA performed on purified HisTag-PCSK9 recombinant protein. A serial dilution of Biotin Anti- HisTag Rabbit Monoclonal Antibody Clone RM146 was used as the primary antibody, followed by alkaline phosphatase conjugated Streptavidin.
Western blot of 293T cells transfected (+) or untrans- fected (-) with a DNA construct encoding His-Tag Histone H3 (G34W) protein, using Biotin Anti-HisTag Rabbit Monoclonal Antibody Clone RM146 at 0.2 ug/mL, followed by a HRP conjugated Streptavidin.
Immunohistochemistry staining of naïve HepG2 cells (Negative control) using anti-His-Tag antibody, RM146.