Anti-Trimethyl Histone H3 (Lys9) rabbit monoclonal antibody [RM389]
Datasheet
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Catalog#: 31-1275-00
Applications: WB, ELISA, Multiplex
Price Not Available
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Product Details
Product Specifications |
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Product Name |
Anti-Trimethyl Histone H3 (Lys9) rabbit monoclonal antibody [RM389] |
| Product Description: |
Rabbit monoclonal to Dimethylated Histone H3 Lysine 9, H3K9me2 |
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Catalog No. |
31-1275-00 |
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Clone Name |
RM389 |
| Product Datasheet |
Anti-Trimethyl Histone H3 (Lys9) rabbit monoclonal antibody [RM389] |
| Specificity |
This antibody reacts to Histone H3 trimethylated at Lysine 9 (K9me3). No cross reactivity with monomethylated Lysine 9 (K9me1), dimethylated Lysine 9 (K9me2), or other methylations in histone H3. |
|
Immunogen |
A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys9). |
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Application |
WB, ELISA, Multiplex |
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Species Reactivity |
Human Vertebrates |
| Conjugate |
None |
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Intended Use |
Research Use Only |
Properties |
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Host |
Rabbit |
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Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1.0 mg/mL |
Volume/Size |
Anti-Trimethyl Histone H3 (Lys9) rabbit monoclonal antibody [RM389] |
| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
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Clone Type |
Recombinant Monoclonal |
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Isotype |
Rabbit IgG |
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Uniport |
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Gene ID |
8350 |
| Usage |
Western Blot: 0.5 ug/mL-2 ug/mL;ELISA: 1 ug/mL - 10 ug/mL;Multiplex: 0.5 ug/mL – 2 ug/mL. |
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Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM389 at 0.5 ug/mL, showed a band of histone H3 trimethylated at Lysine 9 (K9me3) in HeLa cells.
ELISA of Designer Nucleosomes (dNucs) (Recombinant Human Nucleosome with H3 K4, 9, 27, 36 and H4 K20 Mono-, Di-, and Tri-Methylation), using anti-H3K9me3 rabbit monoclonal antibody RM389 (0.5 μg/mL).