Anti-Trimethyl-Histone H3 (Lys4) rabbit monoclonal antibody [RM137]
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Catalog#: 31-1039-00
Applications: WB, ELISA, Multiplex
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Product Details
Product Specifications |
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Product Name |
Anti-Trimethyl-Histone H3 (Lys4) rabbit monoclonal antibody [RM137] |
Product Description: |
Rabbit monoclonal to Trimethylated Histone H3 Lysine 4, H3K4me3 |
Catalog No. |
31-1039-00 |
Clone Name |
RM137 |
Product Datasheet |
Anti-Trimethyl-Histone H3 (Lys4) rabbit monoclonal antibody [RM137] |
Specificity |
This antibody reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with monomethylated Lysine 4 (K4me1) or dimethylated Lysine 4 (K4me3), or other methylation in histone H3. |
Immunogen |
A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys4) |
Application |
WB, ELISA, Multiplex |
Species Reactivity |
Human Vertebrates |
Conjugate |
None |
Intended Use |
Research Use Only |
Properties |
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Host |
Rabbit |
Storage Instruction |
Store at -20.0°C |
Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
Anti-Trimethyl-Histone H3 (Lys4) rabbit monoclonal antibody [RM137] |
Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
Clone Type |
Recombinant Monoclonal |
Isotype |
Rabbit IgG |
Uniport |
|
Gene ID |
8350 |
Usage |
Western Blot: 0.2 ug/mL – 1 ug/mL;ELISA: 0.1 ug/mL – 0.5 ug/mL;Multiplex: 0.1 ug/mL – 0.5 ug/mL. |
Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
A Peptide dotblot shows RM137 reacts only to Histone H3 trimethyl-Lysine 4 (K4me3). No cross reactivity with nonmodified Lysine 4 (H3N1-19), monomethylated Lysine 4 (K4me1) or dimethylated Lysine 4 (K4me2).
RM137 specifically reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with other methylated lysines in Histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM137 at 0.5 ug/mL, showed a band of histone H3 tri- methylated at Lysine 4 (K4me3) in HeLa cells.