Anti-Trimethyl-Histone H3 (Lys27) rabbit monoclonal antibody [RM175]
Datasheet
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Catalog#: 31-1062-00
Applications: WB, ELISA, Multiplex, IHC
Price Not Available
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Product Details
Product Specifications |
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Product Name |
Anti-Trimethyl-Histone H3 (Lys27) rabbit monoclonal antibody [RM175] |
| Product Description: |
Rabbit monoclonal to Trimethylated Histone H3 Lysine 27, H3K27me3 |
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Catalog No. |
31-1062-00 |
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Clone Name |
RM175 |
| Product Datasheet |
Anti-Trimethyl-Histone H3 (Lys27) rabbit monoclonal antibody [RM175] |
| Specificity |
This antibody reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity with monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in histone H3. |
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Immunogen |
A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys27) |
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Application |
WB, ELISA, Multiplex, IHC |
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Species Reactivity |
Human Vertebrates |
| Conjugate |
None |
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Intended Use |
Research Use Only |
Properties |
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Host |
Rabbit |
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Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
Anti-Trimethyl-Histone H3 (Lys27) rabbit monoclonal antibody [RM175] |
| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
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Clone Type |
Recombinant Monoclonal |
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Isotype |
Rabbit IgG |
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Uniport |
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Gene ID |
8350 |
| Usage |
Western Blot: 1 ug/mL – 2 ug/mL;IHC: 0.5 ug/mL – 2 ug/mL;ELISA: 0.5ug/mL - 1 ug/mL;Multiplex: 0.1 ug/mL – 0.5 ug/mL. |
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Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
RM175 specifically reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity with non-modified Lysine 27 (K27 Ctrl), monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in Histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM175 at 1 ug/mL, showed a band of histone H3 trimethyl- ated at Lysine 27 (K27me3) in HeLa cells.
Immunohistochemistry staining of HepG2 cells using Anti-Trimethyl-Histone H3 (Lys27) antibody, RM175.