Product Details
Product Specifications |
|
---|---|
Product Name |
Anti-Human IgG4 rabbit monoclonal antibody [RM120] |
Product Description: |
Rabbit monoclonal to Human IgG4 |
Catalog No. |
31-1022-00 |
Clone Name |
RM120 |
Product Datasheet |
|
Specificity |
This antibody reacts to the heavy chain of human IgG4, RM120 does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG3), and shows no cross reactivity to IgM, IgA, IgD, IgE. RM120 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. |
Immunogen |
Peptide corresponding to the hinge region of Human IgG4 |
Application |
WB, FC, ELISA |
Species Reactivity |
Human |
Conjugate |
None |
Intended Use |
Research Use Only |
Properties |
|
---|---|
Host |
Rabbit |
Storage Instruction |
Store at -20.0°C |
Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
|
Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
Clone Type |
Recombinant Monoclonal |
Isotype |
Rabbit IgG |
Uniport |
|
Gene ID |
3503 |
Usage |
ELISA: 50ng/well – 200ng/well (for Capture);0.05ug/mL – 0.2ug/mL (for Detection);Western Blot (WB): 0.5 ug/mL – 2 ug/mL; |
Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Immunohistochemical staining of formalin fixed and paraffin embedded human lymphoid tissue section using anti-human IgG4 rabbit monoclonal antibody (Clone RM120).
Western Blot of human, mouse, rat, and goat IgG shows RM120 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.
A titer ELISA using RM120. The plate was coated with different amounts of human IgG4. A serial dilution of RM120 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.