Anti-Histone H3 (Unmodified Lys4) rabbit monoclonal antibody [RM186]
Datasheet
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Catalog#: 31-1086-00
Applications: WB, ELISA, Multiplex, ICC
Price Not Available
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Product Details
Product Specifications |
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Product Name |
Anti-Histone H3 (Unmodified Lys4) rabbit monoclonal antibody [RM186] |
| Product Description: |
Rabbit monoclonal to Histone H3 Unmodified Lysine 4 |
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Catalog No. |
31-1086-00 |
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Clone Name |
RM186 |
| Product Datasheet |
Anti-Histone H3 (Unmodified Lys4) rabbit monoclonal antibody [RM186] |
| Specificity |
This antibody recognizes Histone H3 that is unmodified at Lys4 but does not recognize acetylated or methylated Lys4. The antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3. |
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Immunogen |
An unmodified peptide corresponding to the N-terminus of Histone H3. |
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Application |
WB, ELISA, Multiplex, ICC |
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Species Reactivity |
Human Vertebrates |
| Conjugate |
None |
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Intended Use |
Research Use Only |
Properties |
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Host |
Rabbit |
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Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
Anti-Histone H3 (Unmodified Lys4) rabbit monoclonal antibody [RM186] |
| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
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Clone Type |
Recombinant Monoclonal |
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Isotype |
Rabbit IgG |
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Uniport |
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Gene ID |
8350 |
| Usage |
Western Blot: 1 ug/mL – 2 ug/mL;ELISA: 0.5 ug/mL - 1 ug/mL;Multiplex: 0.5 ug/mL – 2 ug/mL. |
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Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody (clone RM186) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Western Blot of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant histone H3.1 protein, using RM186 at 1 ug/mL, showed a band of histone H3 with unmodified Lys4.
RM186 specifically recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, mono- methylated, dimethylated, or trimethylated Lys4. The antibody binding specificity allows for modifications of Arg2 or Thr3 in histone H3.