Anti-Dimethyl Histone H3 (Lys9) rabbit monoclonal antibody [RM151]
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Catalog#: 31-1059-00
Applications: WB, ELISA, Multiplex, ChIP, ICC
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Product Details
Product Specifications |
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Product Name |
Anti-Dimethyl Histone H3 (Lys9) rabbit monoclonal antibody [RM151] |
Product Description: |
Rabbit monoclonal to Dimethylated Histone H3 Lysine 9, H3K9me2 |
Catalog No. |
31-1059-00 |
Clone Name |
RM151 |
Product Datasheet |
Anti-Dimethyl Histone H3 (Lys9) rabbit monoclonal antibody [RM151] |
Specificity |
This antibody reacts to Histone H3 dimethylated at Lysine 9 (K9me2). No cross reactivity with monomethylated Lysine 9 (K9me1) or trimethylated Lysine 9 (K9me3), or other methylation in histone H3. |
Immunogen |
A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys9). |
Application |
WB, ELISA, Multiplex, ChIP, ICC |
Species Reactivity |
Human Vertebrates |
Conjugate |
None |
Intended Use |
Research Use Only |
Properties |
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Host |
Rabbit |
Storage Instruction |
Store at -20.0°C |
Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
Anti-Dimethyl Histone H3 (Lys9) rabbit monoclonal antibody [RM151] |
Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
Clone Type |
Recombinant Monoclonal |
Isotype |
Rabbit IgG |
Uniport |
|
Gene ID |
8350 |
Usage |
Western Blot: 0.25 ug/mL – 1 ug/mL;ChIP: 2 ug/mL- 10 ug/mL;ICC: 0.5 ug/mL - 2 ug/mL;ELISA: 0.2 ug/mL - 1 ug/mL;Multiplex: 0.1 ug/mL – 0.5 ug/mL. |
Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Immunocytochemistry of HeLa cells treated with sodium butyrate, using Dimethyl-Histone H3 (Lys9) Rabbit mAb RM151 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM151 at 0.5 ug/mL, showed a band of histone H3 dimethylated at Lysine 9 (K9me2) in HeLa cells.
RM151 specifically reacts to Histone H3 dimethylated at Lysine 9 (K9me2). No cross reactivity with non -modified Lysine 9 (H3 1-19), monomethylated Lysine 9 (K9me1), trimethylated Lysine 9 (K9me3), or other methylations in Histone H3.