Anti-Dimethyl-Histone H3 (Lys4) rabbit monoclonal antibody [RM135]
Datasheet
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Catalog#: 31-1037-00
Applications: WB, ELISA, Multiplex, ICC
Price Not Available
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Product Details
Product Specifications |
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Product Name |
Anti-Dimethyl-Histone H3 (Lys4) rabbit monoclonal antibody [RM135] |
| Product Description: |
Rabbit monoclonal to Dimethylated Histone H3 Lysine 4, H3K4me2 |
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Catalog No. |
31-1037-00 |
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Clone Name |
RM135 |
| Product Datasheet |
Anti-Dimethyl-Histone H3 (Lys4) rabbit monoclonal antibody [RM135] |
| Specificity |
This antibody reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with monomethylated Lysine 4 (K4me1) or trimethylated Lysine 4 (K4me3), or other methylation in histone H3. |
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Immunogen |
A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys4) |
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Application |
WB, ELISA, Multiplex, ICC |
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Species Reactivity |
Human Vertebrates |
| Conjugate |
None |
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Intended Use |
Research Use Only |
Properties |
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Host |
Rabbit |
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Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
Anti-Dimethyl-Histone H3 (Lys4) rabbit monoclonal antibody [RM135] |
| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
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Clone Type |
Recombinant Monoclonal |
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Isotype |
Rabbit IgG |
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Uniport |
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Gene ID |
8350 |
| Usage |
Western Blot: 0.02 ug/mL – 0.2 ug/mL;ICC: 1 ug/mL - 2 ug/mL;ELISA: 0.2 ug/mL - 1 ug/mL;Multiplex: 0.1 ug/mL – 0.5 ug/mL. |
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Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Immunocytochemistry of HeLa cells treated with sodium butyrate, using Dimethyl-Histone H3 (Lys4) Rabbit mAb RM135 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
RM135 specifically reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with mono- methylated Lysine 4 (K4me1), trimethylated Lysine 4 (K4me3), or other methylations in histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM135 at 0.025 ug/mL, showed a band of histone H3 dimethylated at Lysine 4 (K4me2) in HeLa cells.