Anti-Acetyl-Histone H3 (Lys56) rabbit monoclonal antibody [RM179]
Datasheet
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Catalog#: 31-1078-00
Applications: WB, ELISA, Multiplex, ChIP, ICC, IHC
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Product Details
Product Specifications |
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|---|---|
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Product Name |
Anti-Acetyl-Histone H3 (Lys56) rabbit monoclonal antibody [RM179] |
| Product Description: |
Rabbit monoclonal to Acetylated Histone H3 Lysine 56 (K56ac) |
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Catalog No. |
31-1078-00 |
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Clone Name |
RM179 |
| Product Datasheet |
Anti-Acetyl-Histone H3 (Lys56) rabbit monoclonal antibody [RM179] |
| Specificity |
This antibody reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with other acetylated Lysines in histone H3. |
|
Immunogen |
An acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys56). |
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Application |
WB, ELISA, Multiplex, ChIP, ICC, IHC |
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Species Reactivity |
Human Vertebrates |
| Conjugate |
None |
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Intended Use |
Research Use Only |
Properties |
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Host |
Rabbit |
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Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
Anti-Acetyl-Histone H3 (Lys56) rabbit monoclonal antibody [RM179] |
| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
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Clone Type |
Recombinant Monoclonal |
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Isotype |
Rabbit IgG |
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Uniport |
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Gene ID |
8350 |
| Usage |
Western Blot: 1 ug/mL - 2 ug/mL;ICC: 0.5 ug/mL - 2 ug/mL;IHC: 1 ug/mL – 10 ug/mL;ELISA: 0.5 ug/mL - 1 ug/mL;Multiplex: 0.5 ug/mL – 2 ug/mL. |
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Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate and (2) recombinant histone H3.3, using RM179 at 1 µg/mL, showed a band of histone H3 acetylated at Lysine 56 in treated HeLa.
RM179 specifically reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl), acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.
Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys56) Rabbit mAb RM179 (red). Actin filaments have been labeled with fluorescein phalloidin (green).