ZymoBIOMICS™ Spike-in Control I (High Microbial Load)

Highlights

Absolute Quantification: enables absolute cell number measurements using Next-Generation Sequencing
in situ Quality Control: ensures each sample is quantified accurately
Unique composition: comprised of two microbes alien to the human microbiome

Description

ZymoBIOMICS™ Spike-in Control I (High Microbial Load) consists of equal cell numbers of two bacteria strains, Imtechella halotolerans and Allobacillus halotolerans. When spiked into an unknown sample, this product will serve as an in situ positive control for DNA-sequencing-based (e.g. NGS-based) microbiome measurements. These two bacteria, Imtechella halotolerans (Gram-negative) and Allobacillus halotolerans (Gram-positive) represent different cell recalcitrance and can expose potential bias during DNA extraction. Moreover, with accurately quantified cell numbers, this standard enables absolute cell number quantification in microbiome measurements.

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Technical Specifications

Biosafety	This product is not biohazardous as microbes have been fully inactivated.
Impurity Level	< 0.01% foreign microbial DNA
Reference Genomes	Complete reference genomes and 16S rRNA genes
Sample Storage	-80°C
Storage Solution	DNA/RNA Shield

Citations

ZymoBIOMICS Spike-in Control I (High Microbial Load)

Cat #	Name	Size
D6320	ZymoBIOMICS Spike-in Control I (High Microbial Load)	25 Preps
D6320-10	ZymoBIOMICS Spike-in Control I (High Microbial Load)	250 Preps

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