ZymoBIOMICS HMW DNA Standard

ZymoBIOMICS HMW DNA Standard

Highlights


  • Long-Read Sequencing Ready: Ideal to benchmark 3rd generation sequencing and metagenomic measurements (e.g. Oxford Nanopore & PacBio).
  • High Molecular Weight: DNA standard is >50 kb in size.
  • Accurate Composition: Composition is cross-validated with multiple types of measurements.


Description


ZymoBIOMICS HMW DNA Standard is a mixture of high molecular weight genomic DNA isolated from pure cultures of seven bacterial and one fungal strains. It has an accurately defined composition, negligible impurities (<0.01%) and contains genomes of a wide range of GC content (15%-85%). This can be used to assess and benchmark the performance of long read/3rd generation sequencing for microbiomics and metagenomics analysis and can also be used as a routine quality control. Theoretical Composition Based on Genomic DNA: Pseudomonas aeruginosa – 14%, Escherichia coli – 14%, Salmonella enterica – 14%, Enterococcus faecalis – 14%, Staphylococcus aureus – 14%, Listeria monocytogenes – 14%, Bacillus subtilis – 14%, Saccharomyces cerevisiae – 2%.

Technical Specifications


Impurity Level <0.01% foreign microbial DNA
Sample Storage -20°C
Source Seven bacteria (3 gram-negative and 4 gram-positive) and 1 yeast.
Storage Solution 10 mM Tris-HCl and 0.1 mM EDTA, pH 8.0

Citations


ZymoBIOMICS HMW DNA Standard

Cat # Name Size
D6322 ZymoBIOMICS HMW DNA Standard 5000 ng

Documents


Protocol: 
Datasheet: 
SDS (MSDS):