Yeast Protein Kit

Yeast Protein Kit

Highlights

  • Convenient: Rapid method for efficient lysis of yeast for downstream protein and DNA analyses.
  • Versatile: Procedure suitable for any fungal species susceptible to Zymolyase.
  • Effective Spheroplasting: Ideal protocol for Western blotting and PCR.


Description

The Yeast Protein Kit is a simple and convenient method for the rapid and thorough lysis of yeast cells. The kit has been optimized for use with S. cerevisiae and C. albicans but can be used for any fungal species that is susceptible to yeast lytic enzyme digestion. The digestion procedure effectively generates spheroplasts of yeast cells, making them ideal for both protein and DNA analyses including Western blotting and PCR, respectively.

Technical Specifications

Applicable For Spheroplasts are ideal for both protein and DNA analyses including Western blotting and PCR, respectively.
Equipment Microcentrifuge & Heat Block/Bath
Processing Time 35 Minutes
Sample Source Cell Culture (Colonies/Patches or Liquid Culture)

Product FAQ

Q1: What strains is this kit compatible with?

Any strains susceptible to Zymolyase, which includes the following fungal genera:

Asbya
Kloekera
Candida
Kluyveromyces
Debaryomyces
Lipomyces
Eremothecium
Metschikowia
Endomyces
Pichia
Hansenula
Pullularia
Hanseniaspora
Saccharomyces
Saccaromycodes
Saccharomycopsis
Schizosaccahromyces
Torulopsis

Citations

Kit Components

Yeast Protein Kit

Cat # Name Size
Y1002 Yeast Protein Kit 200 Preps

Documents

Protocol: 
SDS (MSDS):