highlights

  • Superior activity.
  • Certified RNase-free and protease-free.
  • Withstand more than 10 freeze-thaw cycles.
Description

DNase I is an endonuclease that nonspecifically cleaves single- and double-stranded DNA. It requires divalent metal cations to be active.

Technical Specifications
Unit Definition One Kunitz causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25°C, pH 7.5, when acting on salmon sperm DNA according to the assay method of Kunitz. One Unit of DNase I is equivalent to 12 Kunitz.
Storage After reconstitution, place on ice until ready to use or store frozen aliquots (-20°C).
Reconstitution Add DNase/RNase-free water to the lyophilized DNase I, mix by gentle inversion. Avoid phosphate buffer and calcium chelators.
Inactivation Heat inactivate at 75 °C for 10 min with 5 mM EDTA.

applications

  • Remove DNA from protein and RNA samples.
  • Preparation of DNA-free RNA.
  • DNA labeling by nick-translation.
  • Removal of DNA template after in vitro transcription.
  • Removal of DNA from RNA samples prior to downstream applications (NextGen sequencing, RT-PCR).

kit components

Cat # Name Size
E1010-1-4 DNA Digestion Buffer 4 mL
E1010-1-16 DNA Digestion Buffer 16 mL

DNase I Set

Cat # Name Size
E1010 DNase I Set 250 U
E1011 DNase I Set 1500 U
E1012 DNase I Set 5 x 1500 U
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