Kallikrein 5 Monoclonal Antibody
- Size: 100ul
- Application: WB, FCM, IF(ICC)
- Reactivity: Human
- Predicted Reactivity:
-
Datasheet
Tech Support
IMAGES
A431 cells were fixed,permeabilized and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Kallikrein 5 Monoclonal Antibody(bsm-54746R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (red).
MCF-7 cell;4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Kallikrein 5) monoclonal Antibody, Unconjugated (bsm-54746R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
PRODUCT DETAILS
SPECIFICATIONS |
|
---|---|
PRODUCT NAME |
Kallikrein 5 Monoclonal Antibody |
CONJUGATION |
Unconjugated |
HOST |
Rabbit |
SOURCE |
Synthetic Peptide corresponding to Human Kallikrein 5. |
IMMUNOGEN RANGE |
|
CLONALITY |
Monoclonal |
ISOTYPE |
IgG |
CONCENTRATION |
1ug/ul |
PURIFICATION |
Purified by Protein A. |
STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
|
---|---|
GENE ID |
|
SUBCELLULAR LOCATION |
Secreted |
SYNONYMS |
HK5 antibody, Kallikrein 5 antibody, Kallikrein L2 antibody, Kallikrein like 2 antibody, Kallikrein like protein 2 antibody, Kallikrein related peptidase 5 antibody, Kallikrein-5 antibody, Kallikrein-like protein 2 antibody, Kallikrein5 antibody, KLK 5 antibody, KLK L2 antibody, KLK-L2 antibody, KLK5 antibody, KLK5_HUMAN antibody, KLKL 2 antibody, KLKL2 antibody, SCTE antibody, Stratum corneum tryptic enzyme antibody, UNQ570/PRO1132 antibody |
BACKGROUND |
|
APPLICATION DILUTION |
WB(1:300-1000), FCM(1:20-100), IF(ICC)(1:50-200) |