Tat-SF1 Monoclonal Antibody
- Size: 100ul
- Application: WB, IHC-P, IF(ICC)
- Reactivity: Human, Mouse
- Predicted Reactivity:
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Datasheet
Tech Support
IMAGES
Paraformaldehyde-fixed, paraffin embedded Human thyroid; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tat-SF1 Monoclonal Antibody, Unconjugated (bsm-54743R) at 1:100 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tat-SF1 Monoclonal Antibody, Unconjugated (bsm-54743R) at 1:100 for 30 minutes at room temperature, DAB staining.
RWPE-1 cell;4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Tat-SF1) monoclonal Antibody, Unconjugated (bsm-54743R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tat-SF1 Monoclonal Antibody, Unconjugated (bsm-54743R) at 1:100 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tat-SF1 Monoclonal Antibody, Unconjugated (bsm-54743R) at 1:100 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tat-SF1 Monoclonal Antibody, Unconjugated (bsm-54743R) at 1:100 for 30 minutes at room temperature, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Tat-SF1 Monoclonal Antibody |
CONJUGATION |
Unconjugated |
HOST |
Rabbit |
SOURCE |
Synthetic peptide within N-terminal Human Tat-SF1. |
IMMUNOGEN RANGE |
|
CLONALITY |
Monoclonal |
ISOTYPE |
IgG |
CONCENTRATION |
1ug/ul |
PURIFICATION |
Purified by Protein A. |
STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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GENE ID |
|
SUBCELLULAR LOCATION |
Nucleus |
SYNONYMS |
Cofactor required for Tat activation of HIV 1 transcription antibody, dJ196E23.2 antibody, HIV Tat specific factor 1 antibody, HTATSF1 antibody, HTSF-1 antibody, HTSF1 antibody, TAT SF1 antibody, Tat-SF1 antibody |
BACKGROUND |
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APPLICATION DILUTION |
WB(1:300-1000), IHC-P(1:200-400), IF(ICC)(1:50-200) |