Syntaxin 1a Monoclonal Antibody
- Size: 100ul
- Application: WB, FCM, IHC-P
- Reactivity: Human, Mouse, Rat
- Predicted Reactivity:
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Datasheet
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IMAGES
Paraformaldehyde-fixed, paraffin embedded Human pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Syntaxin 1a Monoclonal Antibody, Unconjugated (bsm-54728R) at 1:200 for 30 minutes at room temperature, DAB staining.
SH-SY5Y cells were fixed,permeabilized and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Syntaxin 1a Monoclonal Antibody(bsm-54728R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (red).
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Syntaxin 1a Monoclonal Antibody, Unconjugated (bsm-54728R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Syntaxin 1a Monoclonal Antibody, Unconjugated (bsm-54728R) at 1:50 for 30 minutes at room temperature, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
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|---|---|
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PRODUCT NAME |
Syntaxin 1a Monoclonal Antibody |
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CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Synthetic Peptide within N-terminal Human Syntaxin 1a. |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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|---|---|
| GENE ID |
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| SUBCELLULAR LOCATION |
Secreted, Cell membrane, Synaptic vesicle membrane, Synaptosome |
| SYNONYMS |
HPC 1 antibody, Neuron specific antigen HPC1 antibody, Neuron-specific antigen HPC-1 antibody, OTTHUMP00000174615 antibody, OTTHUMP00000174616 antibody, OTTHUMP00000174617 antibody, OTTHUMP00000174618 antibody, P35-1 antibody, STX1 antibody, STX1A antibody, STX1A_HUMAN antibody, SYN1A antibody, Syntaxin 1A (brain) antibody, Syntaxin 1A brain antibody, Syntaxin-1A antibody |
| BACKGROUND |
Syntaxin 1a is potentially involved in docking of synaptic vesicles at presynaptic active zones and may play a critical role in neurotransmitter exocytosis. Haploinsufficiency of STX1A may be the cause of certain cardiovascular and musculo skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. |
| APPLICATION DILUTION |
WB(1:300-1000), FCM(1:20-100), IHC-P(1:200-400) |