Rad17 Polyclonal Antibody, Cy5 Conjugated
- Size: 100ul
- Application: IF(IHC-P), IF(IHC-F), IF(ICC)
- Reactivity:
- Predicted Reactivity: Human, Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit
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PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Rad17 Polyclonal Antibody, Cy5 Conjugated |
CONJUGATION |
Cy5 |
HOST |
Rabbit |
SOURCE |
KLH conjugated synthetic peptide derived from human Rad17 |
IMMUNOGEN RANGE |
101-230/681 |
CLONALITY |
Polyclonal |
ISOTYPE |
IgG |
CONCENTRATION |
1ug/ul |
PURIFICATION |
Purified by Protein A. |
STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
STORAGE CONDITION |
Store at 4°C for 12 months. |
TARGET |
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GENE ID |
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SUBCELLULAR LOCATION |
Nucleus |
SYNONYMS |
RFC; CCYC; Cell cycle checkpoint protein; Cell cycle checkpoint protein RAD17; HRAD 17; hRad17; R24L; Rad 17; Rad 24; RAD1 S. pombe homolog; RAD17; RAD17 homolog; Rad17 like protein; RAD17_HUMAN; RAD17Sp; Rad24; RF C activator 1 homolog; RF C/activator 1 homolog; RF-C/activator 1 homolog. |
BACKGROUND |
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013]. |
APPLICATION DILUTION |
IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200) |