PRODUCT NAME

Anti-Mouse NK1.1 In Vivo Antibody - Low Endotoxin (PK136)

SPECIES REACTIVITY

Mouse

HOST

Mouse

SPECIFICITY

Anti-NK1.1 In Vivo Antibody - Low Endotoxin (PK136) recognizes an epitope on Mouse NK1.1

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

Mouse NK cells. Endogenous and interferon induced NK cells. Not present on cytotoxic T-cells and Con A stimulated blast cells. May be present on a certain subset of cytotoxic T-cells strains A, AKR, BALB/C, BALB.H-2b, CBA, C3H, DBA/2, 124 were negative using PK136.

IMMUNOGEN

NK-1+ cells from mouse spleen and bone marrow

CONCENTRATION

FORMULATION

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

PURITY

>95% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 1.0 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 5%

STORAGE CONDITION

anti-NK1.1 In Vivo Antibody - Low Endotoxin (PK136) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

TARGET

NK1.1

CLONE

PK136

SYNONYMS

BACKGROUND

NK-1.1 surface antigen, also known as CD161b/CD161c, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

UNIPROT

PATHOGEN TEST

APPLICATIONS

Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, CyTOF, IHC (Frozen)

APPLICATION NOTES

Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.