PRODUCT NAME

Anti-Mouse IFN gamma In Vivo Antibody - Low Endotoxin (H22)

SPECIES REACTIVITY

Mouse

HOST

Armenian Hamster

SPECIFICITY

Anti-IFN gamma In Vivo Antibody - Low Endotoxin (H22) recognizes an epitope on Mouse IFN-gamma

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

IMMUNOGEN

Purified Recombinant Mouse IFN-gamma (>98%)

CONCENTRATION

FORMULATION

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

PURITY

>95% by SDS-PAGE and HPLC

ENDOTOXIN

<1.0 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 5%

STORAGE CONDITION

anti-IFN gamma In Vivo Antibody - Low Endotoxin (H22) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

TARGET

IFN gamma

CLONE

H22

SYNONYMS

BACKGROUND

Interferon-gamma (IFN-gamma) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-gamma has antiviral, immunoregulatory, and anti-tumour properties

UNIPROT

P01580

PATHOGEN TEST

APPLICATIONS

Immunoprecipitation, Western Blot, Neutralization, ELISA, Immunofluorescence

APPLICATION NOTES

Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.