HOT FIREPol® Blend Master Mix Ready to Load with 10 mM MgCl2
- Ready to load hot-start PCR master mix for more demanding PCR assays
Robust and reliable hot-start Master Mix with higher fidelity and longer amplification range for more demanding reactions. Includes two tracking dyes.
✓ increased yield, sensitivity and specificity
✓ up to 5x higher fidelity
✓ suitable for templates up to 5 kb
✓ reduced primer dimer formation
✓ load directly on gel after PCR
Catalog# 04-25-00S20
Size:0.1ml (25 rxn) 25 rxn Free sample
Size | Price |
0.1ml (25 rxn) | Get Quote |
OVERVIEW
A ready-to-use 5X-concentrated solution for more demanding endpoint PCR assays. In addition to the hot-start Taq polymerase HOT FIREPol® this master mix contains a proofreading enzyme which offers enhanced fidelity and performance. The mix has regular and Ready-to-Load version and different MgCl₂ options for easier optimization. Ready-to-Load mix ncludes loading dyes for direct loading to gel. |
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DESCRIPTION |
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HOT FIREPol® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water), additional compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis. |
KITS COMPONENT |
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• HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start |
STORAGE & SHIPPING |
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Storage: Routine storage: -20ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product |
REAGENT PROVIDED |
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CITATION
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