Human Heat Shock 70kDa Protein 1B, HSPA1B ELISA Kit
Product Summary
• Size: 48T,96T
• Sensitivity: 0.098ng/ml
• Detection range: 0.2-70ng/ml
• Reactive with: Human
Product Details
Product Specifications |
|
---|---|
Full Product Name: |
Heat Shock 70Kda Protein 1B |
Catalog No.: |
BL6896EH |
Size: |
48T,96T |
Detaction Range: |
0.2-70ng/ml |
Sensitivity: |
0.098ng/ml |
Sample Type: |
Serum, plasma, cell culture supernates |
Assay Type: |
Sandwich |
Species: |
Human |
Storage: |
2-8ºC |
Assay Time: |
1h 30m |
Background: |
Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The co-chaperones have been shown to not only regulate different steps of the ATPase cycle, but they also have an individual specificity such that one co-chaperone may promote folding of a substrate while another may promote degradation. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. The co-chaperones are of three types: J-domain co-chaperones such as HSP40s (stimulate ATPase hydrolysis by HSP70), the nucleotide exchange factors (NEF) such as BAG1/2/3 (facilitate conversion of HSP70 from the ADP-bound to the ATP-bound state thereby promoting substrate release), and the TPR domain chaperones such as HOPX and STUB1 (PubMed: 24012426, PubMed: 26865365, PubMed: 24318877). Maintains protein homeostasis during cellular stress through two opposing mechanisms: protein refolding and degradation. Its acetylation/deacetylation state determines whether it functions in protein refolding or protein degradation by controlling the competitive binding of co-chaperones HOPX and STUB1. During the early stress response, the acetylated form binds to HOPX which assists in chaperone-mediated protein refolding, thereafter, it is deacetylated and binds to ubiquitin ligase STUB1 that promotes ubiquitin-mediated protein degradation (PubMed: 27708256). Regulates centrosome integrity during mitosis, and is required for the maintenance of a functional mitotic centrosome that supports the assembly of a bipolar mitotic spindle (PubMed: 27137183). Enhances STUB1-mediated SMAD3 ubiquitination and degradation and facilitates STUB1-mediated inhibition of TGF-beta signaling (PubMed: 24613385). Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation (PubMed: 23973223). (Microbial infection) In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. |
UniProt: |
|
MASS(Da): |
70,052 |
GeneID: |
33033304 |
Synonyms: |
Heat shock 70 kDa protein 1B;Heat shock 70 kDa protein 2;HSP70.2;HSP70-2;HSPA1B |
Gene Names: |
HSPA1B |
Research Area: |
Neuroscience |
Target Protein: |
HSPA1B |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Components
Components | Quantity |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard solution | 0.5ml x1 |
Standard diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop solution | 6ml x1 |
Substrate solution A | 6ml x1 |
Substrate solution B | 6ml x1 |
Wash buffer concentrate | (25x) 20ml x1 |
Biotinylated antibody | 1ml x1 |
Documents
Technical Guide
Technical Guide: Human Heat Shock 70kDa Protein 1B, HSPA1B ELISA KitSafety Data Sheets (SDS)
MSDS: Human Heat Shock 70kDa Protein 1B, HSPA1B ELISA KitReferences
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