ZymoBIOMICS DNA Miniprep Kit
D4300
| Cat # | Name | Size |
|---|---|---|
| D4300 | ZymoBIOMICS DNA Miniprep Kit (Lysis Tubes) | 50 Preps |
| D4300T | ZymoBIOMICS DNA Miniprep Kit | 5 Preps |
| D4304 | ZymoBIOMICS DNA Miniprep Kit (No Lysis Matrix) | 50 Preps |
highlights
- ✓ Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
- ✓ Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
- ✓ Certified Low Bioburden: Boost your detection limit for low abundance microbes.
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
|---|---|
| Elution Volume | ≥ 50 µl DNA Elution Buffer or DNase free water |
| Equipment | Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended). |
| Processing Volume | Fecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2×109 bacterial, 2×108 yeast, or 2×107 mammalian cells) |
| Purity | High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8 |
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms, and water. |
| Sample Storage | Eluted DNA should be stored at ≤ -20°C. |
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. |
| Type | Total DNA |
| Yield | Up to 25 µg total DNA can be eluted into ≥50 µl |
Video
Kits Component
| Cat # | Name | Size |
|---|---|---|
| C1058-50 | Zymo-Spin III-HRC Filters | 50 Pack |
| S6012-50 | ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) | 50 Tubes |
| C1078-50 | Zymo-Spin IICR Columns | 50 Pack |
| D4300-1-40 | ZymoBIOMICS Lysis Solution | 40 ml |
| D4300-2-100 | ZymoBIOMICS DNA Binding Buffer | 100 ml |
| D4300-3-50 | ZymoBIOMICS DNA Wash Buffer 1 | 50 ml |
| D4300-7-30 | ZymoBIOMICS HRC Prep Solution | 30 ml |
| D4300-4-60 | ZymoBIOMICS DNA Wash Buffer 2 | 60 ml |
| D4302-5-1 | ZymoBIOMICS DNase/RNase Free Water | 1 ml |
| D4302-5-10 | ZymoBIOMICS DNase/RNase Free Water | 10 ml |
| C1057-50 | Zymo-Spin III-F Filters | 50 Pack |
reviews
Based on 74 reviews
Trusted reviews provided by
Archana Kumari
Rowan University School of Osteopathic Medicine
Jul 28 2021
Easy to use.
Achieved high quality results, easy to use.
Application Area: DNA isolation from ChIP samples
Matthew Brock
UT Southwestern Medical Center
Jul 28 2021
This extraction kit works great and is very easy to use.
This kit is very easy to use and works great for most microbial samples. We use it mostly for bacterial extraction from stool samples. The gDNA collected afterward is great for 16s sequencing or shotgun metagenomics. This is our go to kit for most samples.
Application Area: Microbiome extraction
Isis Mejias
Global WaSH
Jul 28 2021
Fast and reliable results.
The kit is very easy to use and effective. This is my number 1 kit. Instructions to use are very clear and concise.
Application Area: DNA extraction
Neha Mittal
University of North Carolina at Charlotte
Jul 28 2021
Amazing quality results/kit/chemicals.
This kit has a great combination of chemicals that can provide good quality and quantity. Easy to use with straightforward protocol to be use. I use it almost everyday and very happy with its performance. Highly recommended.
Application Area: Extraction kit
Zhuoteng Yu
Precidiag Inc
Jul 28 2021
Easy to handle, good results.
This DNA/RNA Kit is not very consistent for the faecal microbiome. RNA output is too low to detect. But it is easy to handle.
Application Area: Analyzing microbiome DNA