Catalog#: 31-1099-02
Applications: WB (nonreduced only), IP, ICC, IHC, FC, ELISA
Price Not Available
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Product Details
Product Specifications |
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|---|---|
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Product Name |
Biotin Anti-Mouse IgA rabbit monoclonal antibody [RM220] |
| Product Description: |
Biotinylated Rabbit monoclonal to Mouse IgA |
|
Catalog No. |
31-1099-02 |
|
Clone Name |
RM220 |
| Product Datasheet |
|
| Specificity |
This antibody specifically reacts to mouse IgA. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA. |
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Immunogen |
Mouse IgA |
|
Application |
WB (nonreduced only), IP, ICC, IHC, FC, ELISA |
|
Species Reactivity |
Mouse |
| Conjugate |
Biotin |
|
Intended Use |
Research Use Only |
Properties |
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|---|---|
|
Host |
Rabbit |
|
Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
|
| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
|
Clone Type |
Recombinant Monoclonal |
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Isotype |
Rabbit IgG |
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Uniport |
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Gene ID |
238447 |
| Usage |
ELISA: 0.005 ug/mL – 0.2 ug/mL;Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL; Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL; Western Blot (WB): 0.5 ug/mL-2 ug/mL. |
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Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
ELISA of mouse immunoglobulins shows RM220 reacts to mouse IgA. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM220 was used as the primary antibody. An alkaline phosphatase conjugated anti- rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgA. The plate was coated with different amount of mouse IgA. A serial dilution of RM220 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Western blot of nonreduced(-) and reduced(+) mouse IgA, using 0.5ug/mL of RevMAb clone RM220. This antibody reacts to nonreduced IgA.