Description
KEY FEATURES:• Amine-reactive; has a spacer with arm length of 22.4 Å which helps to minimize steric hindrance to biotin labeling• More water-soluble than the corresponding NHS compound without the sulfo group• Membrane-impermeable• Forms permanent amide bonds and the spacer arm cannot be cleavedAPPLICATIONS:• Sulfo-NHS-LC-Biotin is ideal for biotinylating antibody or protein that can then be used for protein detection, purification or immobilization • Sulfo-NHS-LC-Biotin is particularly useful for targeting cell surface proteins due to the membrane-impermeability of this reagent
Datasheet
SKU-Size | 2326-set (Size: 1 set (10 x 1 mg)) 2326-50 (Size: 50 mg) 2326-100 (Size: 100 mg) |
---|---|
Alternate Name | Sulfosuccinimidyl 6-(biotinamido) Hexanoate; Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt |
Appearance | White to Off-white Solid |
CAS # | 127062-22-0 |
Molecular Formula | C₁₄H₁₈O₈N₃S₂Na |
Molecular Weight | 556.59 |
Purity | ≥90% by NMR |
Solubility | H₂O (~ 20 mg/ml) |
SMILES | O=C([MH]1)NC2C1CSC2CCCCC(NCCCCCC(ON3C(C(S(=O)(O[Na])=O)CC3=O)=O)=O)=O |
InChi | InChI=1S/C20H30N4O9S2.Na/c25-15(7-4-3-6-13-18-12(11-34-13)22-20(29)23-18)21-9-5-1-2-8-17(27)33-24-16(26)10-14(19(24)28)35(30,31)32;/h12-14,18H,1-11H2,(H,21,25)(H2,22,23,29)(H,30,31,32); |
InChi Key | RDSJVNGBJUJRRW-UHFFFAOYSA-N |
PubChem CID | 16219001 |
MDL Number | MFCD00078534 |
Handling | Protect from moisture |
Storage Conditions | -20 ̊C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not For Use in Humans. |
FAQ
When a protein is biotinylated by Sulfo-NHS-LC-Biotin, which part of this molecule attached to protein?
Sulfo-NHS-LC-biotin (Sulfosuccinimidyl 6-(biotinamido) Hexanoate) is an amine-reactive biotinylation agent. It covalently binds to the amine groups in proteins and other molecules through its NHS ester forming an amide linkage.
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact Tech Support
High Quality Guaranteed Product
Our products such as Elisa, Antibodies, Proteins, Peptides and sequencing kits are covered by Biolinkk quality warranty and will work as described in datasheet, a free replacement or money back is guaranteed if does not perform according to datasheet.
Learn More