Guanidine Thiocyanate (GITC)

Product Description:

Guanidine Thiocyanate (Guanidinium thiocyanate or guanidinium isothiocyanate, GITC), a powerful protein denaturant, is most often used to inactivate endogenous RNases in the isolation of RNA from various tissues and bacteria. When used with acid-equilibrated phenol or phenol: chloroform, a solution of Guanidine Thiocyanate and beta-mercaptoethanol are very effective in disrupting both cytoplasmic and nuclear membranes while maintaining the integrity of the RNA. Guanidine Thiocyanate is also used as a storage buffer for blood samples. Guanidine Thiocyanate from Bio Basic has been manufactured and specially packaged to provide a higher purity nuclease-free product.

CAS:

[593-84-0]

Total Product Size: 

250g/1kg

Individual Container Size:

1

Number of Containers:

1

Grade:

Biotechnology

DG:

Yes

Storage:

18 to 25C

Sterile:

No

Shipping Conditions:

RT

UNSPSC Code:

12352200

UNSPSC Category:

Biochemical Reagents

More information:

Hazard Class: 8 Hazard UN: 1759 Hazard PG: PGIIIReferences:1. Chomczynski, P. and N. Sacchi. 1987. Anal. Biochem. 162:156-159. 2. Kedzierski, W. and J.C. Porter. 1991. BioTechniques. 10:210-214. 3. Chirgwin, J.M., et.al., 1979. Biochem. 18:5294. 4. Maniatis, T., et. al., 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Press.