- Ready-to-use, ultra pure 5-Hydroxymethylcytosine dCTP; >99% trisphosphate as determined by HPLC analysis.
- Readily incorporates into PCR amplicons with ZymoTaq or other DNA polymerases.
- Free of endo/exo-deoxyribonucleases, ribonucleases, and phosphatases.
5-Hydroxymethylcytosine dCTP can be used in nucleotide mixtures to generate PCR amplicons using ZymoTaq, QuestTaq, or other DNA polymerases.
|Applicable For||PCR amplicons containing 5-methylcytosine are useful in a variety of applications such as studying 5-methylcytosine binding/interacting proteins or defining methods that distinguish between 5-methylcytosine and 5-hydroxymethylcytosine.|
|Concentration||5-Hydroxymethyl dCTP: 10µmol/100 µl as a [100 mM] solution.|
|Sample Storage||5-Hydroxymethylcytosine dCTP is guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.|