- One Input, One Column: Purify RNA and DNA into separate fractions with just one spin-column, directly from a sample in TRIzol, TRI Reagent® or a similar acid-guanidinium-phenol based reagent.
- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- High-Quality: DNA, RNA (including small/miRNAs) is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc.
Upon lysis of the sample with TRIzol or similar, RNA and DNA is bound directly to the Zymo-Spin™ Column. Then simply spin, wash, and elute high-quality RNA and DNA into separate fractions. No phase separation, precipitation, or post-purification steps are necessary. The eluted nucleic acids are suitable for all subsequent molecular manipulations and analyses including Next-Gen sequencing, RT/qPCR, hybridization, etc.
|Binding Capacity Specification||25 µg DNA and 50 µg RNA (yield will vary with type and treatment of sample)|
|Compatibility||Aqueous (RNA) phase from TRIzol, TRI Reagent® or similar can be processed. Also, compatible with samples in DNA/RNA Shield™ (see Appendix, page 5)|
|Elution Volume||≥ 25 µl|
|Purity||DNA and RNA ready for all subsequent downstream analyses (Next-Gen sequencing, RT/qPCR)|
|Sample Inactivation||TRI Reagent® (provided with R2081, R2083 only) inactivates viruses and other infectious agents.|
|Sample Type Specifications||Cells (animal, bacterial, yeast), tissue (animal, plant) or biological liquids (blood, plasma, serum, CSF, buffy coat, etc.), freshly lysed in TRIzol, TRI Reagent® or similar.|
|Size Range||up to and above 40 kb (DNA), ≥ 17 nt (RNA)|
Direct-zol DNA/RNA Miniprep