Fibronectin (16E5) Monoclonal Antibody
- Size: 100ul
- Application: WB, IP
- Reactivity: Human
- Predicted Reactivity:
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Datasheet
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IMAGES
Immunoprecipitation of FibronectinÊfrom human serum using Fibronectin (16E5) Monoclonal Antibody (bsm-50208M). Lane 1 : Human Serum (0.5uL); Lane 2 : Precipitated from 1ul of Human Serum using 2ug of antibody; Lane 3 : Precipitated from 1ul of Human Serum using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50208M at 1.0ug/mL (1:1000) and incubated at 4_ overnight, followed by secondary antibody incubation for 60min at room temperature.
Lane 1 : Human Serum 0.5uL; Lane 2 : Human Serum 1.0uL; probed with bsm-50208M Fibronectin (16E5) Monoclonal Antibody at 1.0ug/mL (1:1000) dilution and incubated at 4_ overnight, followed by secondary antibody incubation for 60min at room temperature.
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Fibronectin (16E5) Monoclonal Antibody |
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CONJUGATION |
Unconjugated |
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HOST |
Mouse |
| SOURCE |
Protein purified from Human plasma |
| IMMUNOGEN RANGE |
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| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG1 |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein G. |
| STORAGE BUFFER |
HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. |
| STORAGE CONDITION |
Store for 1 year at -20°C from date of shipment. Store for 1 year at -20°C from date of shipment. |
TARGET |
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| GENE ID |
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| SUBCELLULAR LOCATION |
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| SYNONYMS |
FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; Fibronectin; Cold-insoluble globulin; FN1 |
| BACKGROUND |
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. |
| APPLICATION DILUTION |
WB(1:300-1000), IP(1-2ug) |