Bioss Antibodies check tests
FIREScript® KIT (06-13-00050)
Fast and robust reverse transcriptase for wide range of applications
Genetically modified MMLV-based reverse transcriptase with increased
thermostability and improved performance at elevated temperatures
* High specificity and yield
* Wide reaction temperature from 37°C to 60°C
* Fast 15 min reaction time
* A simple 2-vial kit with enzyme and reaction buffer only
* Reaction set-up and shipment without ice
FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase.
This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C-60°C.
FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).
Filixic acid ABA, N2396
Luciferin, IPTG, and Competent Cells
Molecular Biology Reagents and Tools: Luciferin, IPTG, and Competent Cells
Molecular biology relies heavily on a wide range of reagents and tools to explore genetic processes and manipulate biological systems. Luciferin, IPTG, and Competent Cells are fundamental products used in various molecular biology applications. Luciferin, a bioluminescent substrate, is essential for reporter assays and gene expression studies, enabling real-time detection of biological activity. IPTG (Isopropyl β-D-1-thiogalactopyranoside) is a key inducer used to trigger gene expression in bacterial systems, particularly in the lac operon system, facilitating protein production. Competent Cells, treated bacterial cells capable of taking up foreign DNA, are indispensable for genetic transformation, cloning, and the creation of recombinant DNA. These products are integral to advancing molecular biology research and experimentation.
Luciferin
IPGT
Competent Cell
Bioluminescence
Luciferin
GoldBio provides a premier selection of luciferin substrates for bioluminescent assays in biological research, supporting gene network and signaling studies with high-quality products and reliable resources. Our offerings include Firefly D-Luciferin salts, perfect for luciferase imaging in mice, cell cultures, and ATP assays, and Coelenterazine, suited for BRET, ELISA, and chemiluminescent detection of reactive oxygen species. GoldBio’s Illumination™ Assay Kits, available in flash and glow formats and lyophilized for convenience, enable precise firefly and renilla activity measurements. We also provide assay lysis buffers and supporting reagents for a complete research solution.
Luciferin Resources
GoldBio’s complete luciferin resource collection provides detailed guides to support successful luciferase assays. The luciferase assay kit comparison guide and fact sheet offer insights into selecting the best substrate for your system. Additionally, GoldBio provides detailed protocols for Illumination™ assay kits, including the Firefly & Renilla Enhanced Assay, along with in vitro and in vivo D-Luciferin handbooks for precise luciferase activity measurement in cell cultures and animal models.