The Minute™ kit is a next-generation, spin column–based system designed for rapid and efficient total protein extraction from animal cultured cells and tissues. Unlike conventional solution-based methods, such as RIPA buffer, which often result in protein loss and altered profiles, this patented technology preserves protein integrity and yields reproducible results.
With enhanced lysis buffers and a streamlined protocol, proteins can be extracted in just 1–5 minutes at concentrations ranging from 1–8 mg/ml. The system supports both denaturing and native conditions, allowing users to choose based on downstream applications. Minimal extraction volume (as low as 20 µl) makes it ideal when starting material is limited.
This kit offers one of the fastest protocols available for total protein preparation and can be readily adapted for high-throughput workflows. The extracted proteins are also suitable as starting material for small-scale purification using column chromatography.
-RIPA Buffer – The Potential Troubles
-Pitfalls of Protein Extraction by RIPA
-A Technical Breakthrough for Protein Sample Preparation
Selected references related to protein loss by RIPA buffer:
Protein profiles of animal tissues extracted by denaturing lysis buffer and native lysis buffer. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1, drosophila larvae; lane 2, drosophila pupa; 3, adult drosophila; 4, goldfish muscle and 5, mouse liver.
| Items | Quantity |
|---|---|
| Denaturing Cell Lysis Buffer | 25 ml |
| Native Cell Lysis Buffer | 25 ml |
| Protein Extraction Filter Cartridges | 50 units |
| Collection Tubes with Caps | 50 units |
| Plastic Rods | 2 units |