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Home / Sample Preparation Kits / Cell Fractionation & Protein Trafficking / Minute™ Plasma Membrane-Derived Lipid Raft Isolation Kit

Minute™ Plasma Membrane-Derived Lipid Raft Isolation Kit (20 preps)

Cat# : LR-042

Description

Lipid rafts are small membrane domains containing a high level of cholesterol and sphingolipids. Lipid rafts have been found in the plasma membrane (PM) and internal organellar membranes such as mitochondria-associated membrane (MAMs) and endoplasmic reticulum. Lipid rafts are implicated in numerous cellular processes such as signal transduction, membrane trafficking, and protein sorting.  Lipid-modified proteins and some transmembrane proteins are concentrated in the rafts while other proteins are excluded. Lipid rafts are also found to be associated with Na+/K+ ATPase on PM. Traditional methods for lipid raft isolation involve isolation of detergent-resistant membrane subdomain from total membranous structures, which does not distinguish plasma membrane-derived and/or organelle-derived lipid rafts. Using the patented spin-column-based technologies, we have developed this kit specifically for the isolation of plasma membrane-derived lipid rafts. Larger plasma membrane vesicles are first isolated and treated with a non-ionic detergent containing buffer followed by isolation of detergent-resistant fraction by flotation centrifugation using a tabletop microcentrifuge. Highly enriched plasma membrane-derived lipid rafts can be obtained in about 1 hour without using a traditional homogenizer and ultracentrifugation.

*For total lipid raft isolation, please refer to MinuteTM Total Lipid Raft Isolation Kit under Cat # LR-039.

Kit Components:

Items

Quantity

Buffer A

15 ml

Buffer B

10 ml

Buffer C

10 ml

Plastic Rods

2 units

Filter Cartridge with Collection Tubes

20 units
 References (13)
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  7. Gao, S. S., Shi, R., Sun, J., Tang, Y., Zheng, Z., Li, J. F., … & Li, C. (2022). GPI-Anchored Ligand-BioID2-Tagging System Identifies Galectin-1 Mediating Zika Virus Entry. iScience, 105481.
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