Description
Sensitive and highly specific cDNA synthesis and qPCR in a single tube.
Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip® RNase inhibitor, and nuclease-free water.
* cDNA synthesis up to 60°C for superior specificity
* Analyze 1-4 targets in 1 reaction
* Reaction set-up and shipment without dry ice
* Robust amplification of GC-rich targets
* Contains dUTP to prevent cross-contamination when used in combination with UNG
* RNase inhibitor included in kit
SOLIScript® 1-step Multiplex Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube.
Reagent composition is optimized for excellent performance in highly multiplexed reactions while making sure you don’t lose in sensitivity compared to singleplex or duplex assays.
Application
Detection and quantification of RNA targets with qPCR;
DNA/LNA hydrolysis probe-based assays;
Suitable for singleplex and multiplex reactions
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