HOT FIREPol® EvaGreen® HRM Mix (no ROX), Solis BioDyne

Home PCR qPCR and RT-qPCR reagents qPCR and RT-qPCR HRM HOT FIREPol® EvaGreen® HRM Mix (no ROX)

HOT FIREPol® EvaGreen® HRM Mix (no ROX)

For research use only.

Some applications of this product may require a license which is not provided by the purchase of this product.

Ordering Information

Catalog Number	Size & Unit
08-31-0000S	0.2ml (50 rxn)
08-31-00001	1ml (250 rxn)
08-31-00001-5	5ml (5-pack: 5 x 1 ml; 1250 rxn)
08-31-00001-10	10ml (10-pack: 10 x 1 ml; 2500 rxn)
08-31-00020	20ml (5000 rxn)

Description

Optimized ready-to-use solution for High Resolution Melt (HRM) Analysis.
* Highly sensitive mix for accurate SNP detection
* Sensitive EvaGreen® dye for reliable HRM performance
* Reaction set-up without dry ice

High-Resolution Melt (HRM) analysis is a fast and cost-effective method for post-PCR analysis to detect single nucleotide polymorphisms (SNP genotyping), genetic mutations, and DNA sequence variations.

5x HOT FIREPol® EvaGreen® HRM Mix is an optimized ready-to-use solution for HRM analysis, incorporating EvaGreen® dye.

It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2, and EvaGreen® dye. The user simply needs to add water, template, and primers.

HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers.

Application

High-Resolution Melt (HRM);
SNP detection;
dye-based qPCR

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HOT FIREPol® EvaGreen® HRM Mix (no ROX)

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