Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Gigaprep kit provides the fastest and simplest method available to efficiently isolate up to 25 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a patented binding system, the ZymoPURE II Gigaprep kit can process up to 2.5 L of culture in less than 45 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is rapidly bound onto a column with a vacuum instead of a slow gravity flow column. Additionally, there is no alcohol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 6 µg/µl), endotoxin-free and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Gigaprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.