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Protein extraction from cell-wall-containing microbes is a common procedure. The methods for protein extraction are usually harsh, tedious and unreliable. YT-015 provides an instrument-free, fast and gentle way for extracting proteins from microbes with thick and strong cell walls. These microbes include yeast, filamentous fungus, gram-positive and -negative bacteria, insect eggs, microalgae and more. This kit contains optimized denaturing and native protein extraction buffers to choose from. Unlike many other methods with harsh conditions, such as 8 M urea, glass bead lysis using an instrument and boiling the sample with alkaline extraction, etc., are used for yeast protein extraction. Proteins in certain molecular weight ranges are usually preferentially extracted with these methods. This kit features a single tube protocol and allows for a full range of yeast proteins to be extracted without bias. The kit works well for both log-phase and stationary-phase microbes. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 2-5 mg/ml. The materials provided are sufficient for 50 extractions.

Passive elution and electroelution are the most common methods for extracting proteins/nucleic acid from polyacrylamide/agarose gels. Protein extraction by passive elution usually takes overnight incubation. Eluted proteins are significantly diluted and require further concentration. Protein extraction by electroelution is faster (30 minutes to 2 hours) than passive elution, but it requires a special elution device that works less effectively for larger proteins (>70 kDa). The electro buffer usually contains detergent and other chemicals at a concentration that may interfere with downstream applications. Here we feature a rapid and instrument-free protein/nucleic acid extraction kit. Proteins/nucleic acid can be extracted from gels in less than 10 minutes with a high yield. The elution buffer can be a detergent-containing buffer or pure water depending on the method of gel staining. The elution volume is between 10 and 200 µl. Multiple gel pieces can be processed in a single tube, and the final protein concentration is relatively high.

Histones and other DNA-binding proteins play an important role in chromosome organization, gene regulation and post-translational modification. Core components of histones can be covalently modified through methylation, acetylation, phosphorylation and sumoylation. These modifications are essential for gene expression, regulation, DNA repair and chromosome condensation. Minute™ Histone/DNA Binding Protein Extraction Kit is designed to efficiently extract histones and other DNA-binding proteins from cultured cells and isolated cells. Unlike many other commercial histone extraction kits that employ acid or high-salt extraction methods, Minute™ Histone/DNA Binding Protein Extraction Kit is efficient in extracting histones and other DNA-binding proteins that can be used for internal controls for histone modification analysis. In comparison, acid extraction protocol that selectively extracts basic proteins and a good internal control is usually not possible. Minute™ Histone/DNA Binding Protein Extraction Kit can extract histones from 0.5 to 5 million cells in less than 10 minutes with high protein yield (1-2.5 mg/ml), making it the fastest and most robust histone extraction kit available on the market.

Despite significant variation in body organization, insects all have the same general body structure. They have segmented bodies divided into three regions: head, thorax and abdomen. The body segments are protected by a hard exoskeleton or cuticles. From a protein extraction point of view, the unique structure of an exoskeleton makes it very hard to homogenize. It is also very difficult to lyse cells protected by cuticle for total protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the protein yield is low. The profile of extracted protein using traditional methods is usually incomplete. This kit provides a highly efficient method for total protein extraction from insects using a combination of mechanical extraction and chemical lysis. The cell lysis buffers used are much stronger than RIPA buffers. The kit features a simple, fast single-tube protocol and optimized buffers for insect tissues. Researchers have the option to choose either a denaturing or native cell lysis buffer, which is specifically tailored for insects. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 1-3 mg/ml. The materials provided are sufficient for 50 extractions.

Shipping and storage: This kit is shipped and stored at room temperature.

Skeletal and cardiac muscles are highly specialized tissues that are made of well-arranged muscle fibers.  An individual muscle may be made up of hundreds or even thousands of muscle fibers bundled together and wrapped in a connective tissue covering. The unique organization of the muscle tissue makes it very hard to homogenize. It is also very difficult to lyse cells in the tissue for total protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the protein yield is low. The profile of extracted protein using traditional methods is usually incomplete. This kit provides a highly efficient method for total protein extraction from human/animal skeletal or cardiac muscle tissues using a combination of mechanical extraction and chemical lysis. The cell lysis buffers used are much stronger than RIPA buffers. The kit features a simple, fast single-tube protocol and optimized buffers for muscle tissues. Researchers have the option to choose either a denaturing or native cell lysis buffer, which is specifically tailored for muscle tissues. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 2-10 mg/ml. The materials provided are sufficient for 50 extractions.

Shipping and storage: This kit is shipped and stored at room temperature.

The intestines are a long and continuous tube that run from stomach to anus. Mesentery is a fold of tissue that attaches intestines to the body wall. Structurally, intestines and mesentery are very different. However, from a protein extraction point of view, they have one thing in common: they are very tough and hard to homogenize. It is also very difficult to lyse cells in the tissues for total protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the protein yield is very low. The profile of extracted protein using traditional methods is usually incomplete. This kit provides a highly efficient method for total protein extraction from human or animal intestines and mesentery tissues using a combination of mechanical extraction and chemical lysis. The cell lysis buffers used are much stronger than RIPA buffers. The kit features a simple, fast single-tube protocol and optimized buffers for intestine and mesentery tissues. Researchers have the option to choose either a denaturing or native cell lysis buffer for specific downstream applications. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 1-3 mg/ml. The materials provided are sufficient for 50 extractions.

Shipping and storage: This kit is shipped and stored at room temperature.

A tendon/ligament is a fibrous connective tissue that attaches muscles to bone. Histologically tendons consist of dense regular collagen fibers that are very tough and notoriously difficult to homogenize. It is also very difficult to lyse the tendon tissues for total protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the protein yield is very low. The profile of extracted protein is also biased (incomplete protein profile) with solution-based methods. This kit provides a highly efficient method for total protein extraction from human or animal tendons by a combination of mechanical extraction and chemical lysis. The cell lysis buffers used are much stronger than RIPA buffers. The kit features a simple, fast single-tube protocol and optimized buffers for tendons. Researchers have the option to choose either a denaturing or native cell lysis buffer, which is specifically tailored for tendons. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 1-3 mg/ml. The materials provided are sufficient for 50 extractions.

Shipping and storage: This kit is shipped and stored at room temperature.

Blood vessels (arteries and veins) are made of specialized connective tissues. There are three layers of tissues in blood vessels: tunica, tunica media and endothelium. Due to their unique structures, blood vessels are notoriously difficult to homogenize. It is also very difficult to lyse the cells in the tissue for total protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the protein yield is very low. The profile of extracted protein is also biased with solution-based (incomplete protein profile) methods. This kit provides a highly efficient method for total protein extraction from human or animal blood vessels by a combination of mechanical extraction and chemical lysis. The cell lysis buffers used are much stronger than the RIPA buffer. The kit features a simple, fast single-tube protocol and optimized buffers for blood vessels. Researchers have the option to choose either a denaturing or native cell lysis buffer, which is specifically tailored for blood vessels. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 1-3 mg/ml. The materials provided are sufficient for 50 extractions.

Shipping and storage: This kit is shipped and stored at room temperature.