Many crop species across the plant family contain high polysaccharide and polyphenols. The hydrocolloid mucilage is a complex mucopolysaccharide typically showing high viscosity when released in solution. Homogenate of high mucopolysaccharide plant samples usually imparts a slimy consistency that is most unwanted contaminants that interfere with cell fractionation and organelle purification, such as chloroplast and nuclear isolation. This kit provides a simple, rapid, and efficient method for isolating chloroplasts from viscous plant homogenate. The protocol takes about 30 min.
Figure 1. Chloroplasts isolated from leaves of indicated plant species.
Figure 2. Morphologies of chloroplasts isolated from leaves of indicated plants under light microscopy.
Kit Components:
| Buffer A | 30 ml |
| Buffer B | 30 ml |
| Micro Puncher | 2 |