Human Methylated & Non-Methylated (WGA) DNA Set (DNA w/ Primers)- Zymo Research Corporation

Human Methylated & Non-Methylated

Cat #	Name	Size
D5013	Human Methylated & Non-Methylated (WGA) DNA Set (DNA w/ Primers)	5 ug/20 ul

Original Manufacturer: Zymo Research

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Highlights

Purified, non-methylated and methylated human DNA for use as negative and positive control in methylation detection applications.
Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency.
Ideal controls for bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP). Primers included with the full set are designed to amplify non-methylated, methylated, and mixed methylated copies of the Ras association (RalGDS/AF-6) domain family member 1 (RASSF1) gene following bisulfite conversion.

Product Description

The Human WGA Methylated & Non-methylated DNA Set consists of two control DNAs (non-methylated and methylated) along with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation, EZ DNA Methylation-Gold, EZ DNA Methylation-Direct, and EZ DNA Methylation-Lightning kits from Zymo Research to assess the efficiency of bisulfite-mediated conversion of DNA. The Human WGA Methylated & Non-methylated DNA Set is generated using phi29 DNA polymerase based whole genome amplification techniques from HCT116 DKO cell line derived genomic DNA (Human HCT116 DKO Non-methylated DNA). The Human WGA Methylated DNA is Human WGA Non-methylated DNA that has been enzymatically methylated at all double-stranded CG dinucleotides using M.SssI methyltransferase and can be used as a positive control for DNA methylation analysis.

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Technical Specifications


Name	Human Methylated & Non-Methylated
Catalog	D5013
Applicable For	Nucleic Acid Purification, Zymo Research: Epigenetics, Epigenetics: Standards
Storage	-20C
Self Life	12
Shipping	Blue Ice

Documents

R1141