Read over 350 inspirational, peer-reviewed publications and discover the power of RPA.
Visual and equipment-free reverse transcription recombinase polymerase amplification method for rapid detection of foot-and-mouth disease virus.
Author: Liu L, Wang J, ZhangR, Lin M, Shi R, Han Q, Wang J, Yuan W.
A visible and equipment-free reverse-transcription recombinase polymerase amplification assay combined with lateral flow strip (LFS RT-RPA) was developed to detect the FMDV using primers and LF probe specific for the 3D gene.
A sample-in-digital-answer-out system for rapid detection and quantitation of infectious pathogens in bodily fluids.
Author: Yang H, Chen Z, Cao X, Li Z, Stavrakis S, Choo J, deMello AJ, Howes PD, He N.
An integrated sample-in-digital-answer-out (SIDAO) diagnostic system incorporating DNA extraction and digital recombinase polymerase amplification, which enables rapid and quantitative nucleic acid analysis from bodily fluids within a disposable cartridge.
Development of propidium monoazide-recombinase polymerase amplification (PMA-RPA) assay for rapid detection of Streptococcus pyogenes and Streptococcus agalactiae.
Author: Chen J, Wang Y, Liu X, Chen G, Chen X, Chen J, Liu Z, Gong J, Yang G, Lan Q.
A reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed to detect TMEV infection. The sensitivity of the RT-RPA assay approached 8 copies per reaction, which is equivalent to the sensitivity of RT-qPCR reactions.
Development of propidium monoazide-recombinase polymerase amplification (PMA-RPA) assay for rapid detection of Streptococcus pyogenes and Streptococcus agalactiae.
Author: Chen J, Wang Y, Liu X, Chen G, Chen X, Chen J, Liu Z, Gong J, Yang G, Lan Q.
To determine whether propidium monoazide (PMA) can eliminate the influence of DNA extracted from dead cells, a bacterial suspension was treated with PMA before DNA extraction. Findings of RPA assay showed that DNA extracted from dead cells had no fluorescence …
Naked eye detection of the Mycobacterium tuberculosis complex by recombinase polymerase amplification-SYBR green I assays.
Author: Singpanomchai N, Akeda Y, Tomono K, Tamaru A, Santanirand P, Ratthawongjirakul P.
Rapid diagnosis of Mycobacterium tuberculosis (Mtb) is key to controlling the spread of tuberculosis, which is a global health concern. In this study, isothermal recombinase polymerase amplification (RPA) was developed to detect specific targets of Mtb, IS6110 and IS1081. Additionally, …
A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the bovine respirovirus 3.
Author: Zhao G, Wang H, Hou P, Xia X, He H.
In this study, a novel isothermal assay for detecting BPIV3 was established. The method includes a lateral flow dipstick (LFD) assay combined with reverse transcription recombinase polymerase amplification (RT-RPA).
Data for designing two isothermal amplification assays for the detection of root-infecting fungi on cool-season turfgrasses.
Author: Karakkat BB, Hockemeyer K, Franchett M, Olson M, Mullenberg C, Koch PL.
The data provided here describe the information for designing primers and probes for LAMP and RPA, how specific they are for each of the three fungi, and the evaluation of RPA on field samples.
Arch-shaped multiple-target sensing for rapid diagnosis and identification of emerging infectious pathogens.
Author: Koo B, Hong KH, Jin CE, Kim JY, Kin SH, Shin Y.
In this study, an arch-shaped amplification containing primer sequences was designed to rapidly amplify multiple targets. Further, the sensing platform allowed for sensitive and specific detection of human coronavirus, Middle East respiratory syndrome, Zika virus, and Ebola virus down to …
PrimedRPA: Primer design for Recombinase polymerase amplification assays.
Author: Higgins M, Ravenhall M, Ward D, Phelan J, Ibrahim A, Forrest MS, Clark TG, Campino S.
PrimedRPA is a python-based package that automates the creation and filtering of RPA primers and probe sets. It aligns several sequences to identify conserved targets, and filters regions that cross react with possible background organisms.
Single-Step Recombinase Polymerase Amplification Assay Based on a Paper Chip for Simultaneous Detection of Multiple Foodborne Pathogens.
Author: Ahn H, Batule BS, Seok Y, Kim MG.
A paper chip device-based recombinase polymerase amplification (RPA) method was developed for highly sensitive and selective single-step detection of foodborne pathogens. The diagnostic utility of the device was demonstrated by the reliable detection of E. coli and S.aureus present in …