Author: Wu T, Wu H, Zhao K, Hu C, Ge Y, Zhu X, Zhang X, Zhou M, Zhu F, Cui L.
As an important component of the causative agent of respiratory tract infections, enteric and eye infections, Human mastadenoviruses (HAdVs) species B spread easily in the crowd. In this study, an RPA assay was developed for rapidly detecting HAdVs species B …
Author: Pang J, Wang Q, Fei Y, Zhu P, Qiao L, Huang H, Dang C, Gao W.
Vibrio harveyi is a pathogen that infects fish and shellfish worldwide, causing severe economic losses for the aquaculture industry. As the early diagnosis of V. harveyi infection is crucial to disease surveillance and prevention in cultured marine animals, a fast …
Author: Li TT, Wang JL, Zhang NZ, Li WH, Yan HB, Li L, Jia WZ, Fu BQ.
Accurate diagnosis of Trichinella spp. infection in domestic animals is crucial for the effective prevention and control of human trichinellosis. In the present study, a simple, rapid and accurate diagnostic assay was developed combining recombinase polymerase amplification and a lateral …
Author: Villamor DEV, Eastwell K.
Recent escalation of WX disease in Washington State prompted the development of a rapid detection assay based on recombinase-polymerase amplification (RPA) to facilitate timely removal and replacement of diseased trees. This study reports a reliable RPA assay targeting putative immunodominant …
Author: Zeng R, Luo J, Gao S, Xu L, Song Z, Dai F.
Reverse transcription recombinase polymerase amplification (RT-RPA) for detection of Cucumber green mottle mosaic virus (CGMMV) was developed in this study. It was found to be specific, with a limit of detection of 0.5 pg of total CGMMV RNA. CGMMV on inoculated …
Author: Gunaratna G, Manamperi A, Bohlken-Fascher S, Wickremasinge R, Gunawardena K, Yapa B, Pathirana N, Pathirana H, de Silva N, Sooriyaarachchi M, Deerasinghe T, Mondal D, Ranasinghe S, Abd El Wahed A.
In this study, a mobile suitcase laboratory applying novel extraction (SpeedXtract) and isothermal amplification and detection (recombinase polymerase amplification assay, RPA) methods were evaluated for the diagnosis of cutaneous leishmaniasis in Sri Lanka.
Author: Dey S, Koo K, Wang Z, Sina AAI, Wuethrich A, Trau M.
This study presents an integrated multi-molecular sensor (IMMS) for an entire sample-to-answer workflow from melanoma cell capture to simultaneous quantification of both intracellular BRAFV600E DNA and protein levels on a single platform.
Author: Zhao G, Hou P, Huan Y, He C, Wang H, He H.
Mycoplasma bovis (M. bovis) is a major etiological agent of bovine mycoplasmosis around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equipment. …
Author: Geng Y, Liu G, Lui L, Deng Q, Zhao L, Sun XX, Wang J, Zhao B, Wang J.
In this study, a real-time RPA assay was developed so as to achieve the rapid and efficient detection of C. jejuni by targeting the hipO gene. The specificity and sensitivity of real-time RPA was validated and the practical applicability of …
Author: Ma L, Zeng F, Cong F, Huang B, Zhu Y, Wu M, Xu F, Yuan W, Huang R, Guo P.
Murine norovirus (MNV) is recognized as the most prevalent viral pathogen in captive mouse colonies. The rapid detection assay for MNV would be a useful tool for monitoring and preventing MNV infection. A recombinase polymerase amplification (RPA) assay was established …