Read over 350 inspirational, peer-reviewed publications and discover the power of RPA.
Development of a recombinase polymerase based isothermal amplification combined with lateral flow assay (HLB-RPA-LFA) for rapid detection of “Candidatus Liberibacter asiaticus”.
Author: Ghosh DK, Kokane SB, Kokane AD, Warghane AJ, Motghare MR, Bhose S, Sharma AK, Reddy MK.
Currently, polymerase chain reaction (PCR) and real time PCR have been the gold standard techniques used for detection of ‘Ca. L. asiaticus’. These diagnostic methods are expensive, require well equipped laboratories, not user-friendly and not suitable for on-site detection of …
Development of real-time reverse transcription recombinase polymerase amplification (RPA) for rapid detection of peste des petits ruminants virus in clinical samples and its comparison with real-time PCR test.
Author: Li Y, Li L, Fan X, Zou Y, Zhang Y, Wang Q, Sun C, Pan S, Wu X, Wang Z.
Current detection of PPRV in clinical samples mainly relies on real-time RT-PCR. Particularly, samples collected from rural area require highly equipped laboratories for screening. A rapid, real-time reverse-transcription recombinase polymerase amplification assay (RT-RPA), employing primers and exo probe, was thus …
Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification.
Author: Kim NY, Oh J, Lee SH, Kim H, Moon JS, Jeong RD.
In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/μl of purified RNA …
Capture, Concentration and Detection of Salmonella in Foods Using Magnetic Ionic Liquids and Recombinase Polymerase Amplification.
Author: Hice SA, Clark KD, Anderson JL, Brehm-Stecher BF.
This study combines magnetic ionic liquid (MIL)-based sample preparation with isothermal amplification and detection of Salmonella-specific DNA using Recombinase Polymerase Amplification (RPA).
Adenoviral detection by recombinase polymerase amplification and vertical flow paper microarray.
Author: Nybond S, Reu P, Rhedin S, Svedberg G, Alfven T, Gantelius J, Svahn HA.
Described in this study is the use of isothermal amplification (RPA) of viral DNA at 37 °C coupled to a paper-based vertical flow microarray (VFM) setup that utilizes a colorimetric detection of amplicons using functionalized gold nanoparticles.
Development of a rapid and visual detection method for Rickettsia rickettsii combining recombinase polymerase assay with lateral flow test.
Author: Qi Y, Shao Y, Rao J, Shen W, Yin Q, Li X, Chen H, Li J, Zeng W, Zheng S, Liu S, Li Y.
Rickettsia rickettsii is the causative agent of Rocky Mountain spotted fever, which is the most severe spotted fever group (SFG) rickettsiosis. In this study a rapid, visual, sensitive and specific method for the detection of R. rickettsii based on RPA …
A Sample-to-Targeted Gene Analysis Biochip for Nanofluidic Manipulation of Solid-Phase Circulating Tumor Nucleic Acid Amplification in Liquid Biopsies.
Author: Koo KM, Dey S, Trau M.
in this study, complex liquid biopsy sample-to-targeted genetic analysis was conducted on a biochip with a 50 copies-detection limit within 30 min. The biochip uniquely integrated electrical lysis and release of cellular targets with minimal processing; nanofluidic manipulation to accelerate …
Development and evaluation of serotype-specific recombinase polymerase amplification combined with lateral flow dipstick assays for the diagnosis of foot-and-mouth disease virus serotype A, O and Asia1.
Author: Wang H, Hou P, Zhao G, Yu L, Gao Y, He H.
Foot-and-mouth disease (FMD) caused by foot-and-mouth disease virus (FMDV) is one of the most highly infectious diseases in livestock, and leads to huge economic losses. In this study, a series of serotype-specific reverse transcription recombinase polymerase amplification assays combined with …
Development of a reverse transcription recombinase polymerase amplification combined with lateral-flow dipstick assay for avian influenza H9N2 HA gene detection.
Author: Wang Z, Yang P, Zhang Y, Tian K, Bian C, Zhao J.
Simple, rapid, sensitive and accurate methods suitable for field detection of H9N2 AIVs are crucial to efficiently control virus infection and spread in time. In this study, an isothermal reverse transcription recombinase polymerase amplification with lateral‐flow dipstick (RT‐RPA‐LFD) assay for …
Reverse transcription-recombinase polymerase amplification combined with lateral flow strip for detection of rice black-streaked dwarf virus in plants
Author: Zhao C, Sun F, Li X, Lan Y, Du L, Zhou T, Zhou Y.
Rice black-streaked dwarf virus (RBSDV) infects rice plants, a major crop, and is transmitted via the small brown planthopper (SBPH: Laodelphax striatellus Fallén), causing significant economic loss in China. To rapidly diagnose RBSDV, a reverse transcription-recombinase polymerase amplification (RT-RPA) method …