Zymoprep Yeast Plasmid Miniprep I

Highlights

  • Simple: Quickly and easily rescue plasmid from yeast.
  • Efficient Isolation: Works well with low-copy and hard to isolate plasmids.
  • High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR, transformation, hybridization, etc.


Description

The Zymoprep Yeast Plasmid Miniprep I is a simple and efficient yeast plasmid miniprep that is based on the E. coli alkaline lysis method but using Zymolyase as the first solution. The kit provides all the necessary reagents for plasmid isolation from S. cerevisiae, C. albicans and S. pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The procedure is simple and efficient, and there is no need for glass beads or phenol. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard to isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR, and Southern blot analysis.

Technical Specifications

Applicable For Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications
Elution Volume = 35 µl
Equipment Microcentrifuge & Heat Block/Bath
Processing Volume = 1.5 ml of Culture
Sample Source Cell Culture (Colonies/Patches or Liquid Culture)
Size Range No Size Limit
Yield Typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures

Product FAQ

Q1: What is the difference between Zymoprep Yeast Plasmid Miniprep I and Miniprep II?

Both the Zymoprep Yeast Plasmid Miniprep I and II utilize the same chemistry for lysis; however, Miniprep I uses isopropanol precipitation and Miniprep II utilizes a column for purification. The Miniprep II allows for consistent yield and purity; and samples can be concentrated to a low elution volume.

Q2: What is the typical plasmid yield?

Typically, between 0.01 – 0.3 ng for most 2 µ based plasmid from 1.5 ml overnight cultures. In order to generate more plasmid, the plasmid is typically transformed into E. Coli , cultured, and isolated using a traditional E. Coli plasmid prep.

Q3: Can this kit be used to isolate linear plasmid DNA?

Yes

Q4: If I’m using stationary phase yeast cells, what can I do to improve sample lysis?

We generally recommend working with fresh or early log phase cells, which are easier to lyse. For stationary phase cells, user optimization is necessary, and we recommend increasing digestion to > 1 hour and/ or increasing the amount of Zymolyase.

Q5: What strains are these kits compatible with?

Any strains susceptible to Zymolyase, which includes the following fungal genera:

Asbya,
Kloekera,
Candida,
Kluyveromyces,
Debaryomyces,
Lipomyces,
Eremothecium,
Metschikowia,
Endomyces,
Pichia,
Hansenula,
Pullularia,
Hanseniaspora,
Saccharomyces,
Saccaromycodes,
Saccharomycopsis,
Schizosaccahromyces,
Torulopsis

Citations

Kit Components

Zymoprep Yeast Plasmid Miniprep I

Cat # Name Size
D2001 Zymoprep Yeast Plasmid Miniprep I 100 Preps

Documents

Protocol: 
Datasheet: 
SDS (MSDS):