ZymoBroth

Growth medium for the preparation of highly competent E.coli cells.

Highlights

  • Prepare Highly Competent E. coli: Increase transformation efficiency up to 100 fold.
  • Improves Most E. coli Strains: ZymoBroth stimulates the transformation efficiency of a wide range of strains, including K12 derivatives (JM109, HB101, etc.) and B strain derivatives (BL21, etc.)
  • Better Results: Ideal growth medium for generating competent cells with difficult-to-transform E. coli strains.


Description

ZymoBroth (ZB) is a specially formulated growth medium used for the preparation of highly competent E. coli cells for DNA transformation. When compared to classic SOB growth medium, ZymoBroth dramatically increases transformation efficiency, typically on the order of 5 – 100 fold (depending on the E. coli strain). As part of our popular Mix & Go! E. coli Transformation Kit, ZB enables researchers to generate their own homemade Mix & Go! E. coli for DNA transformation. ZB medium has been tested on a wide range of E. coli strains. Our data indicate that ZB medium stimulates the transformation efficiency of all E. coli strains tested, including K12 derivatives (such as JM109, HB101, etc.) and B strain derivatives (such as BL21, etc.).

Technical Specifications

Product Storage Room Temperature

Product FAQ

Q1: How does Zymobroth compare to SOB and LB?

E. coli cells prepared with ZymoBroth growth medium exhibit faster transformation kinetics and higher transformation efficiencies. This may be as high as several folds to a log increase in comparison to SOB. Compared to SOB, LB could reduce the transformation efficiency by 2-5 folds. ZymoBroth increases transformation efficiency, typically on the order of 5 to 100-folds for most labs strains.

Q2: I see some precipitation in the ZymoBroth, is it still okay to use?

The ZymoBroth is fine to use. The precipitation is due to salts coming out of solution. Warm the ZymoBroth at 37°C to re-dissolve.

Citations

ZymoBroth

Cat # Name Size
M3015-100 ZymoBroth 100 ml
M3015-500 ZymoBroth 500ml

Documents

Datasheet: 
SDS (MSDS):