ZymoBIOMICS Targeted Sequencing Service: 16S/ITS

3 Page Report


  • Complete Solution: Simply submit samples and receive a comprehensive, user-friendly data report with publication-ready plots and figures.
  • Quality Ensured: A mock microbial community standard is included with each run to ensure accurate data processing.
  • Superior Taxonomic Resolution: Species-level resolution with 16S rRNA gene sequencing.


Precisely and accurately identify microbial composition. The full service includes unbiased DNA extraction (optional), library preparation, post-library QC, sequencing using the Illumina MiSeq® platform (2x300bp), and bioinformatics analysis. All steps of the process, including library preparation, are performed with a microbial standard for quality control. Bioinformatics analysis is performed using an upgraded pipeline with an in-house curated database to resolve most microbes to the species level and provide increased coverage of organisms. The final report includes publication-ready compositional bar plots, taxonomy heat maps, alpha-diversity plots, beta-diversity plots, LEfSe biomarker discovery, and Taxa2SV decomposer analysis.

Technical Specifications

Minimum Samples per Order 10
Sample Preparation • Label sample tubes with numbers (i.e. 1, 2, 3…) that match the Sample Information File. Do NOT label with unique sample names/IDs.

• Ship all samples together.

• Keep an aliquot of each sample at your facility.

• Dilute DNA samples to 5-20 ng/µl microbial DNA. DNA samples must be suspended in = 20 µl of either DNase/RNase-Free Water or TE Buffer (or similar). For projects with = 20 samples, store in 1.5 mL centrifuge tubes. For projects with > 20 samples, store in clear PCR plates. Ship DNA samples on ice.

• Raw samples (e.g. fecal, soil, swab) without preservative solution must be shipped on dry ice. Raw samples stored in DNA/RNA Shield can be shipped at room temperature. No other preservative solution will be accepted.

Sample Report Download Sample Report
Turnaround Time Guaranteed 6-8 weeks; most projects are completed within 4 weeks. Expedited services are available at additional cost. Please contact zymobiomics-services@zymoresearch.com for expedited service requests and for large sample number orders.

Product FAQ

Q1: How do I ship my raw samples?

Raw samples should be shipped frozen in dry ice. Alternately, raw samples could be stored in DNA/RNA Shield reagent which can preserve the microbial profile of samples at room temperature for over 1 month. Multiple collection tubes option is available.

Q2: What is microbial standard, how does it serve as a control?

Microbial standard includes easy-to-lyse and tough-to-lyse bacteria and yeast to ensure the data generated is bias-free and representative of the microbial sample.

Q3: What extraction method does this service use?

ZymoBIOMICS DNA extraction kit is used to guaranteed to provide unbiased lysis of all of the organisms in a sample, including tough-to-lyse organism.

Q4: What primers set does this service use for targeted sequencing?

For the 16S service, an in-house optimized 16S V3-V4 primers set is used by default to introduce coverage for both archaea and bacteria. For the ITS service, an in-house optimized ITS primer set is used by default. Please contact zymobiomics-services@zymoresearch.com if a different primer set is desired.

Q5: What is the taxonomy resolution for this service?

Our upgraded pipeline is able to resolve most microbes to the species level, and our in-house curated database provides increased coverage of organisms.



“We had a positive experience with ZymoBIOMICS Services. They did a good job in terms of report quality, turnaround time, trouble shooting, etc. Overall, we were very satisfied.”

– Beilei G. (FDA)

“Very happy, thank you!”

– Swati C. (Calysta)

“Excellent customer and technical support.”

– Ali K. (California Institute of Technology)

“I had a wonderful experience with Zymo Research and its Microbiomics Services team. One thing that stood out for me was the flexibility of the team and willingness to work with different samples.”

– Gorakh T. (Florida International University)

“My experience with Zymo was extremely smooth. I was incredibly impressed by the professional, yet friendly, manner of their business. I was satisfied with the detailed results they provide, the helpfulness of the staff and the understanding of customer service. It was simple, straightforward and on time.”

– Ashraf B. (The American University in Cairo)

“I really appreciated how helpful the Zymo Research team was throughout this process. Thank you for promptly responding to all of our concerns and answering all our questions.”

– Deepthi T. (Washington & Lee University)

“Excellent customer support in a project involving multiple parties. There were several logistical issues that needed to be addressed and Zymo Research handled it well with good communication. Data delivery and reporting were completed on time and with even more analysis than expected. Will be using Zymo again for similar services!”

– Tom G. (BP)

“Thank you for the quality service!”

– Nakako S. (Shiseido)

“I had a nice experience working with the ZymoBIOMICS Services Team- they helped me with all my requests and were very prompt in responding to emails.”

– Madhuri J. (Thermo Fisher)

“The professionalism and continued communication was wonderful during the entire process. When we received the data back, I was impressed to see that the samples are collected at various cycles once amplification occurs. This something that we have never seen with other sequencing services we have used.”

– Joleah L. (Cornell University)

“The ZymoBIOMICS Service Team is exceptionally helpful and dedicated. They performed custom DNA extraction for our unique applications and made helpful recommendations about sample submission. I have been very pleased with the data and provided services.”

– Jake J. (Troy Corporation)

“The output of the online results is beautiful and very user friendly. ”

– Alicyn R. (Northeastern University)

“The service team is very helpful! I get meaningful results which in the long-run helps me save money by getting the right data from microbiome experiments.”

– Jeff Y. (Blue Owl Brewing)

“I received the results on time and they were presented in a very professional way that we could use immediately. I have reiterate that we are very impressed with the professional way of giving data to customers. With any questions we had, the Zymo Research team was always prompt in replying to them and helped me finish the project very successfully.One month after I received my report, I wrote again for a new analysis of the data with new groupings and within a day I was given again the results, making my data interpretation easy. I thank the Zymo Research team for this wonderful service. I very strongly recommend this company for their excellent customer support and quality of data.”

– Pradheep C. (Maharishi School)

“Working with Zymo Research Microbiomics Services was a very good experience. Lots of data and anlayses were included that I have not received from other services.”

– Karim D. (Florida Atlantic University)

“Zymo Research provides an excellent service with attention to detail. Positive and rewarding.”

– Barry H. (AECOM)

“Excellent experience. The people are so thoughtful and the results are fast and reliable.”

– Mercedes Q. (CINVESTAV)

“Overall I am very pleased in working with Zymo Research and am excited to have the results.”

– Julie H. (North Carolina Museum of Natural Sciences)

“Outstanding from the initial inquiry to receiving the final data report.”

– Kirk B. (Sacred Heart University)

“I was really impressed with the fast turnaround time and the comprehensive analyses included with the report.”

– Carlos G. (North Carolina State University)

“Sequencing is a powerful technology that is constantly evolving because of new applications and biological problems to address. Zymo Research is willing to go out of the way to explore new applications. This means they understand their market and their clients needs.”

– Octavio M. (California Institute of Technology)

“I have being sending samples to ZymoBIOMICS Services for several years and continue to be consistently impressed by the incredible service. The team members are an absolute pleasure to deal with. They have been enormously helpful regarding processing and analysis of samples, and I feel they are always willing to go the extra step to facilitate specifics for my data analysis. Thank you for providing such an awesome service!”

– Susan B. (Dana-Farber Cancer Institute)

“I have used the ZymoBIOMICS Service twice for my Master’s degree. Both times I was very impressed with the services and data provided from the samples. The team does a great job with explaining the results and sequencing details and is always available to answer any questions you may have down the line. I especially appreciated how alpha and beta diversity analyses are part of the results returned (this has saved me a ton of time). The attention and genuine care for the needs of the customer is what makes Zymo stand out as a company and I will definitely recommend them to any colleagues of mine that may need these services in the future. Overall I am very satisfied with Zymo’s services and am even more happy with the customer service.”

– Erick E. (Florida Atlantic Univeristy)

ZymoBIOMICS Targeted Sequencing Service: 16S/ITS

Cat # Name Size
Q2001 16S (With DNA Extraction) 1 sample
Q2012 16S (Without DNA Extraction) 1 sample
Q2003 ITS (With DNA Extraction) 1 sample
Q2013 ITS (Without DNA Extraction) 1 sample