Highlights
- Unbiased Lysis: Efficient and unbiased lysis of microbes including gram positive/negative bacteria, fungi, protozoans, and viruses from any sample including feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.
- Ultra-Pure: High-quality DNA/RNA (including mall/microRNAs) are inhibitor-free and ready for RT/qPCR and microbiome measurements using Next-Gen sequencing.
- High Sensitivity: Increased detection limit of very low abundance organisms.
Description
The ZymoBIOMICS MagBead DNA/RNA kit provides a high-throughput, magnetic bead-based purification of both high-quality DNA and total RNA (including small/microRNAs) from the same starting sample. The provided DNA/RNA Shield inactivates infectious agents and is ideal for sample storage at ambient temperatures. The extraction method utilizes magnetic beads for DNA/RNA extraction without the use of phenol and is eluted into =50 µl of ZymoBIOMICS DNase/RNase-Free Water. DNA/RNA is ready for any downstream application including Next-Gen Sequencing, RT-qPCR,, etc.
Technical Specifications
| Binding Capacity | 15 µg DNA/RNA per 30 µl ZymoBIOMICS MagBinding Beads. |
|---|---|
| Equipment Needed | Magnetic stand or separator, heat block, liquid handler or robotic sample processer (user provided). |
| Purity | High-quality RNA is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc. |
| Recommended Materials | (sold separately) – 96-well Collection Plate (C2002; capacity is up to 1.2 ml/well), 96-Well Block (P1001; capacity is up to 2 ml/well), 96-well Elution Plate (C2003), Cover Foil (C2007), ZR-96 MagStand (P1005). |
| Sample Preservation | DNA/RNA Shield lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures. |
| Sample types | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA are efficiently isolated from = 50 mg of mammalian feces, = 50 mg soil, and 5-20 mg (wet weight) of fungal bacterial cells, biofilms, water, and swabs. |
| Size Limits | Capable of recovering DNA and total RNA = 17 nucleotides. |
| Storage | DNA and RNA eluted with ZymoBIOMICS DNase/RNase-Free Water (provided)can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage. |
Citations
Kit Components
| Cat # | Name | Size |
|---|---|---|
| S6012-50 | ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) | 50 Tubes |
| S6002-96-3 | ZR-96 BashingBead Lysis Rack | 0.5 mm & 0.1 mm |
| E1010 | DNase I Set | 250 U |
| D7010-2-200 | DNA/RNA Prep Buffer | 200 ml |
| D7001-1-200 | DNA/RNA Lysis Buffer | 200 ml |
| D7001-1-50 | DNA/RNA Lysis Buffer | 50 ml |
| D7010-2-50 | DNA/RNA Prep Buffer | 50 ml |
| R2130-1-120 | MagBead DNA/RNA Wash 1 | 120 ml |
| R2130-1-30 | MagBead DNA/RNA Wash 1 | 30 ml |
| R2130-2-20 | MagBead DNA/RNA Wash 2 | 20 ml |
| R2130-2-80 | MagBead DNA/RNA Wash 2 | 80 ml |
| D4302-5-10 | ZymoBIOMICS DNase/RNase Free Water | 10 ml |
| D4302-5-1 | ZymoBIOMICS DNase/RNase Free Water | 1 ml |
| D4302-5-50 | ZymoBIOMICS DNase/RNase Free Water | 50 ml |
| D4302-5-30 | ZymoBIOMICS DNase/RNase Free Water | 30 ml |
| D4302-6-6 | ZymoBIOMICS MagBinding Beads | 6 ml |
| D4302-6-12 | ZymoBIOMICS MagBinding Beads | 12 ml |
| R1100-250 | DNA/RNA Shield | 250 ml |
| R1100-50 | DNA/RNA Shield | 50 ml |
| R1106 | DNA/RNA Shield Collection Tube w/Swab (1 ml fill) | 10 pack |
| R1107 | DNA/RNA Shield Collection Tube w/Swab (1 ml fill) | 50 pack |
| R1101 | DNA/RNA Shield-Fecal Collection Tube | 10 pack |
| R1103 | DNA/RNA Shield Lysis Tubes (Microbe) | 50 pack |
| R1104 | DNA/RNA Shield Lysis Tubes w/Swab (Microbe) | 50 pack |
| R1102 | DNA/RNA Shield Collection Tube | 50 pack |
| D3001-2-20 | Proteinase K w/ Storage Buffer Set | 20 mg |
| D3001-2-5 | Proteinase K w/ Storage Buffer Set | 5 mg |
ZymoBIOMICS MagBead DNA/RNA
| Cat # | Name | Size |
|---|
Documents
Protocol: 
Datasheet:
SDS (MSDS):