ZymoBIOMICS DNA Miniprep Kit

Inhibitor-free microbial DNA from any sample validated for microbiomic and metagenomic analyses


  • Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
  • Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
  • Certified Low Bioburden: Boost your detection limit for low abundance microbes.


The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.

Learn More

Technical Specifications

Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume = 50 µl DNA Elution Buffer or DNase free water
Equipment Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended).
Processing Volume Fecal =200 mg, Soil = 250 mg, Cells = 100 mg (Approximately equal to 2×109 bacterial, 2×108 yeast, or 2×107 mammalian cells)
Purity High quality, inhibitor-free DNA. Typical A260/A280 =1.8
Sample Source Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms, and water.
Sample Storage Eluted DNA should be stored at = -20°C.
Size Range Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
Type Total DNA
Yield Up to 25 µg total DNA can be eluted into =50 µl


Kit Components

ZymoBIOMICS DNA Miniprep Kit

Cat # Name Size
D4300 ZymoBIOMICS DNA Miniprep Kit (Lysis Tubes) 50 Preps
D4300T ZymoBIOMICS DNA Miniprep Kit 5 Preps
D4304 ZymoBIOMICS DNA Miniprep Kit (No Lysis Matrix) 50 Preps


Optimized Lysis Protocols